MULTI-ANTIBIOTICS

ANALYSIS OF MULTI-ANTIBIOTICS IN MILK and YOGHURT LC-MS/MS ANALYSIS KIT

Antibiotics are widely used in veterinary medicine for prevention of disease in food producing animals. Excessive or unconscious usage of antibiotics might result residue in food such as milk, meat, honey and others. These residues can cause toxicity, allergic reactions. In additions low antibiotics residues in food products, consumed for long time and lead to the spread of drug-resistant bacteria. On the other hand, milk goes through production, processing and circulation until reaching consumers.  In order to ensure consumers’ safety and to prevent any harmful health effects, WHO and EU established the MRL for antibiotics. LC-MS/MS has become the method of choice for analysis antibiotics residues due to do its high sensitivity, selectivity, robustness and cost effective.

Jasem method provides straightforward analysis of multi-antibiotics in milk and yoghurt using LC/MS-MS during 22 minutes.

  • Features

    • Analysis of multi-antibiotics in one method

    • No need SPE or immunoaffinity column for extraction

    • Quick and simple sample preparation

  • PARAMETERS

    • Multi-Antibiotics

  • Matrix

    • Milk & Yoghurt

Sample Preparation


  • Step 1

    Pipet 1.0 ml of milk or weight 1.0 g of yoghurt sample into a centrifuge tube.

  • Step 2

    Filter mixture from 0.45 micron RC filter, Directly inject into the system.

  • Step 3

    Add 60 µl of ISTD solution and 2.0 ml Reagent-1 onto sample and vortex for 30 second.

  • Step 4

     Centrifuge at 3000 rpm for 5 min.

  • Total ion chromatogram of antibiotics

  • Antibiotic List for Multi-Antibiotics Kit in Milk

     

    Antibiotics

    LOQ

    (µg/kg)

    Recovery

    (%)

    RSD

    (%)

    1

    5-hyroxy thiobendazole

    0.47

    99

    1.49

    2

    Abamectin

    0.84

    101

    9.39

    3

    Albendazole

    0.12

    106

    1.59

    4

    Ampicillin

    0.78

    101

    5.50

    5

    Amoxicillin

    0.33

    102

    1.91

    6

    Ciprofloxacin

    0.40

    100

    1.54

    7

    Danofloxacin

    0.26

    97

    0.94

    8

    Difloxacin

    0.25

    106

    1.98

    9

    Doxycycline

    0.51

    64

    7.08

    10

    Doramectin

    0.95

    108

    4.74

    11

    Enrofloxacin

    0.29

    103

    1.21

    12

    Epinomectin

    0.55

    98

    8.03

    13

    Erythromycin

    0.45

    104

    4.14

    14

    Fenbendazole

    0.10

    107

    1.74

    15

    Flumequine

    0.12

    104

    2.03

    16

    Ipronidazole

    0.68

    97

    1.75

    17

    Ipronidazole-hydroxy

    1.06

    88

    7.84

    18

    Ivermectin

    0.58

    98

    5.91

    19

    Chlortetracycline

    4.29

    102

    5.26

    20

    Levamisole HCl

    0.22

    115

    1.40

    21

    Marbofloxacin

    0.15

    110

    1.19

    22

    Mebendazole

    0.28

    103

    1.87

    23

    Metronidazole

    0.78

    97

    2.28

    24

    Moxidectin

    0.55

    96

    4.52

    25

    Nalidixic acid

    0.16

    108

    2.19

    26

    Naracin

    0.09

    102

    4.59

    27

    Oxacillin

    3.67

    108

    4.96

    28

    Oxytetracycline

    0.86

    109

    4.73

    29

    Oxfendazole

    0.34

    98

    1.42

    30

    Oxolinic acid

    0.16

    112

    1.58

    31

    Penicillin G

    0.32

    102

    1.93

    32

    Ronidazole

    0.96

    98

    2.52

    33

    Salinomcysin

    0.10

    102

    7.25

    34

    Sarafloxacin

    0.52

    97

    2.09

    35

    Sulfadiazin

    0.32

    95

    3.08

    36

    Sulfadimethoxin

    0.56

    101

    1.58

    37

    Sulfadioxin

    0.29

    100

    2.06

    38

    Sulfachloropyridazine

    1.48

    89

    6.12

    39

    Sulfamerazin

    0.44

    101

    1.36

    40

    Sulfametoxazole

    0.95

    73

    3.70

    41

    Sulfamethazine

    0.54

    97

    1.96

    42

    Sulfathiazole

    0.79

    96

    1.65

    43

    Sulfapyrdine

    0.39

    112

    2.48

    44

    Sulfaquinoxaline

    0.58

    104

    2.24

    45

    Tetracycline

    1.04

    98

    5.30

    46

    Thiabendazole

    0.34

    86

    3.50

    47

    Tilmicosin

    0.48

    111

    1.70

    48

    Tylosin

    0.70

    107

    1.71

    49

    Triclabendazole

    0.83

    108

    4.43

    50

    Toltrazural

    0.11

    102

    4.08

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..

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