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Catecholamines, Free Metanephrines and Serotonin in Urine

Catecholamines, Free Metanephrines and Serotonin in Urine LC-MS/MS Analysis Kit

The major metabolites of catecholamines secreted by the adrenal glands in case of stress are metanephrine, epinephrine, normetanephrine and norepinephrine. These biomarkers are evaluated to determine urinary concentration of them in people with suspected pheochromocytoma. This disease is a very rare tumour and causes hypertension in non-followed cases. It is very important to accurately diagnose before surgical intervention and by looking at the catecholamine derivatives, it is possible to diagnose pheochromocytoma.

Jasem presents this analysis reliably on LC-MS/MS with free metanephrine, catecholamine and serotonin kit in urine.

  • Highlights

    Simultaneous analysis of catecholamines, free metanephrines and serotonin without derivatization.

    Rapid and easy sample preparation; just dilution without SPE

    Including stable labelled isotopes of analyte as internal standards

    7.0 minutes analysis time.
  • Parameters

    Epinephrine, Norepinephrine, Dopamine, Metanephrine, Normetanephrine, 3-metoxytyramine and Serotonin
  • Matrix

    Urine

Sample Preparation


  • Step 1
    Take 50 μl urine sample into a centrifuge tube
  • Step 2
    Add 25 μl of Internal std and 175 μl Reagent-1 then vortex for 15 seconds
  • Step 3
    Centrifuge at 4000 rpm for 5 minutes
  • Step 4
    Decant the clear supernatant into HPLC vial.
  • Chromatogram

  • METHOD PERFORMANCE

    Analytes

    LOQ

    Agilent 6460

    (µg/L)

    LOQ

    Agilent 6470

    (µg/L)

    Accuracy

    Agilent 6470

    (%)

    Repeatability

    Agilent 6470

    (%RSD)

    Linearity

    Agilent 6470

    (R2)

    Epinephrine

    11.04

    0.5

    106

    5,6

    0,996

    Norepinephrine

    20.96

    1.0

    96

    3,6

    0,997

    Dopamine

    36.68

    5.0

    104

    4,2

    0,994

    Metanephrine

    11.9

    1.6

    102

    5,6

    0,995

    Normetanephrine

    12.15

    1.8

    105

    6,4

    0,992

    3-Methoxytyramine

    43.38

    15.0

    107

    4,6

    0,996

    Serotonin

    29.21

    12.0

    96

    2,5

    0,998

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..
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