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Catecholamines, free Metanephrines and Serotonin

CATECHOLAMINES, FREE METANEPHRINES AND SEROTONIN IN URINE LC-MS/MS KIT

The major metabolites of catecholamines secreted by the adrenal glands in case of stress are metanephrine, epinephrine, normetanephrine and norepinephrine. These biomarkers are evaluated to determine urinary concentration of them in people with suspected pheochromocytoma. This disease is a very rare tumour and causes hypertension in non-followed cases. It is very important to accurately diagnose before surgical intervention and by looking at the catecholamine derivatives, it is possible to diagnose pheochromocytoma. Jasem presents this analysis reliably on LC-MS/MS with free metanephrine, catecholamine and serotonin kit in urine.

  • Highlights

    • Simultaneous analysis of catecholamines, free metanephrines and serotonin without
      derivatization rapid and easy sample preparation; just dilution without SPE

    • Including stable labelled isotopes of analyte as internal standards

    • 7.0 min. analysis time

  • Matrix

    • Urine

  • PARAMETERS

    • Epinephrine
    • Norepinephrine
    • Dopamine
    • Metanephrine
    • Normetanephrine
    • 3-Methoxytyramine
    • Serotonin

Sample Preparation

  • Step 1

    Pipette 50 μl urine sample into a centrifuge tube

  • Step 2

    Add 25 μl of Internal std and 175 μl Reagent-1 then vortex for 15 sec.

  • Step 3

    Centrifuge at 4000 rpm for 5 min.

  • Step 4

    Decant the clear supernatant into HPLC vial

  • Urinary catecholamines, free metanephrines and serotonin chromatogram

  • Retention times of the analytes

    Analyte

    Retention Time (min)

    Norepinephrine

    1.76

    Epinephrine

    2.10

    Dopamine

    2.66

    Normetanephrine

    2.47

    3-Methoxytyramine

    3.24

    Metanephrine

    2.81

    Serotonin

    3.14

  • Lineerity & Limit of Quantitation (LOQ)

     

     

    URINE

    Analyte

    R2

    LOQ

    (ng/mL)

    Epinephrine

    0,995

    11.04

    Norepinephrine

    0,994

    20.96

    Dopamine

    0,998

    36.68

    Metanephrine

    0,998

    11.90

    Normetanephrine

    0,995

    12.15

    3-Methoxytyramine

    0,998

    43.38

    Serotonin

    0,997

    29.21

     

  • Repeatability

    Analyte

    RSD %

    Epinephrine

    1.16

    Norepinephrine

    2.28

    Dopamine

    3.82

    Metanephrine

    1.48

    Normetanephrine

    1.59

    3-Methoxytyramine

    2.72

    Serotonin

    2.97

  • Recovery

    Analyte

    Recovery %

    Epinephrine

    105.0

    Norepinephrine

    93.4

    Dopamine

    104.2

    Metanephrine

    115.8

    Normetanephrine

    101.7

    3-Methoxytyramine

    99.1

    Serotonin

    99.4
ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..

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