The major metabolites of catecholamines secreted by the adrenal glands in case of stress are metanephrine, epinephrine, normetanephrine and norepinephrine. These biomarkers are evaluated to determine urinary concentration of them in people with suspected pheochromocytoma. This disease is a very rare tumour and causes hypertension in nonfollowed cases. It is very important to accurately diagnose before surgical intervention and by looking at the catecholamine derivatives, it is possible to diagnose pheochromocytoma.
Jasem presents this analysis reliably on LC-MS/MS with free metanephrine, catecholamine and serotonin kit in urine.
Simultaneous analysis of catecholamines, free metanephrines and serotonin without derivatization.
Rapid and easy sample preparation; just dilution!
7 minutes run.
50 µL of calibrator/QC/urine specimen is transferred to a vial and 25 µL of internal standard is added to the vial, then vortex for 5 sec.
175 µL of Reagent-1 is pipetted to the vial and vortex for 5 sec. prior to injection to LC-MS/MS system.
Analyte |
Retention Time (min) |
Norepinephrine |
1.76 |
Epinephrine |
2.10 |
Dopamine |
2.66 |
Normetanephrine |
2.47 |
3-Methoxytyramine |
3.24 |
Metanephrine |
2.81 |
Serotonin |
3.14 |
|
|
URINE |
Analyte |
R2 |
LOQ |
(ng/mL) |
||
Epinephrine |
0,995 |
11.04 |
Norepinephrine |
0,994 |
20.96 |
Dopamine |
0,998 |
36.68 |
Metanephrine |
0,998 |
11.90 |
Normetanephrine |
0,995 |
12.15 |
3-Methoxytyramine |
0,998 |
43.38 |
Serotonin |
0,997 |
29.21 |
Analyte |
RSD % |
Epinephrine |
1.16 |
Norepinephrine |
2.28 |
Dopamine |
3.82 |
Metanephrine |
1.48 |
Normetanephrine |
1.59 |
3-Methoxytyramine |
2.72 |
Serotonin |
2.97 |
Analyte |
Recovery % |
Epinephrine |
105.0 |
Norepinephrine |
93.4 |
Dopamine |
104.2 |
Metanephrine |
115.8 |
Normetanephrine |
101.7 |
3-Methoxytyramine |
99.1 |
Serotonin |
99.4 |
The easiest way is to fill the contact form. Simply mail us to info@jasem.com.tr or give us a call +902165720200
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