Plasma catecholamines including epinephrine (E), norepinephrine (NE), and dopamine (DA) are used to evaluate adrenosympathetic function in patients. Quantification of plasma catecholamine concentrations is clinically important for the diagnosis of pheochromocytoma and paraganglioma during dynamic clonidine tests. The low circulating concentrations of catecholamines and analytical interferences require tedious sample preparation and long chromatographic runs to ensure their accurate quantification by HPLC with electrochemical detection. The reliable and simultaneous analysis of a broad panel of catecholamines is a powerful tool for the investigation of the hormone status, which is relevant for a variety of clinical questions and diagnoses
Analysis of norepinephrine, epinephrine and dopamine without derivatization
No need of SPE!
Including stable labelled isotopes of each analyte as internal standards
10 minutes analysis time from injection to injection
300 µl of plasma sample is pipetted into the glass tube.
20 µl internal standard is added to the sample and vortexed. Then 600 µl of Reagent-1 is added and swirled for 15 seconds.
1 ml of Reagent-2 is added to the mixture in the same tube. After stirring in the shaker for 3 minutes, it is centrifuged for 2 minutes.
700 µl of the upper phase is pipetted and discarded. Solid Reagent-3 is added over the remaining part in the tube and vortexed for 30 seconds.
200 µl of Reagent-4 and 2 ml of Reagent-5 are added respectively and allow to stir for 7 minutes, then centrifuge at 4000 rpm for 3 minutes.
1.6 ml of the supernatant is evaporated to dryness under nitrogen stream.
Following evaporation, residue is reconstituted using 100 µl of Reagent-1 and vortex 5 seconds prior to analysis.
Analytes |
Retention Times (min) |
Norepinephrine |
3.35 |
Epinephrine |
3.90 |
Dopamine |
4.3 |
|
|
PLASMA |
Compound |
R2 |
LOQ ng/L |
Epinephrine |
0.997 |
29.1 |
Norepinephrine |
0.998 |
38.5 |
Dopamine |
0.995 |
19.3 |
|
Analyte |
Concentration µg/L (plasma) |
CV % |
Epinephrine |
311.8 |
5.3 |
Norepinephrine |
1345 |
6.2 |
Dopamine |
335 |
4.4 |
The easiest way is to fill the contact form. Simply mail us to info@jasem.com.tr or give us a call +902165720200
Necessary cookies are absolutely essential for the website to function properly. This category only includes cookies that ensures basic functionalities and security features of the website. These cookies do not store any personal information.
Any cookies that may not be particularly necessary for the website to function and is used specifically to collect user personal data via analytics, ads, other embedded contents are termed as non-necessary cookies. It is mandatory to procure user consent prior to running these cookies on your website.