METANEPHRINES

METANEPHRINES ANALYSIS IN PLASMA LC-MS/MS KIT

Normetanephrine and metanephrine are the O-methylated derivatives of the catecholamine hormones norepinephrine and epinephrine, respectively. Metanephrines are created by means of catecholamine metabolism but also generated excessively in pheochromocytoma tumors of the chromaffin cells, most of which are localized to the adrenal gland. The majority of patients with a pheochromocytoma tumor have plasma normetanephrine and metanephrine concentrations at least 2–3 times the upper reference limits. The presence of elevated concentrations of the analytes represents as a confirmation tool for the diagnosis of the tumor. In this context, the measurement of free (unconjugated) plasma metanephrines has been proved to be a highly sensitive (With a diagnostic sensitivity of 98% and specificity of 92%) test for the detection of pheochromocytoma.
Jasem kit allows simultaneous quantification of free metanephrines in plasma without using SPE throughout 6.5 minutes.

  • Features

    • Analysis of metanephrine, normetanephrine and 3-metoxytyramine without derivatization

    • No need of SPE! Rapid and easy sample preparation-just protein precipitation!

    • Including stable labelled isotopes of each analyte as internal standards

    • 5 minutes analysis time from injection to injection

  • PARAMETERS

    • Metanephrine
    • Normetanephrine
    • 3-Methoxytyramine
  • MATRIX

    • Plasma

Sample Preparation


  • Step 1

    150 µl of plasma is pipetted to a glass centrifuge tube.

  • Step 2

    20 µl internal standard is added on the glass tube  and vortex for 5 seconds. After that, 250 µl of Reagent-1 is added and vortex for 15 seconds.

  • Step 3

    Centrifuge at 4000 rpm for 5  minutes.

  • Step 4

    Decant the supernatant to a HPLC vial.

  • Extracted ion chromatogram of free metanephrines

  • Calibration Curves

    Normetanephrine

    Metanephrine

  • Linearity & Limit of Quantitation

          

     

    PLASMA

    Analyte

    R2

    LOQ

    (µg/mL)

    Normetanephrine

    0.999

    5.52

    Metanephrine

    0.999

    14.82

    3-Methoxytyramine

    0.998

    3.81

  • Recovery

    Analyte

    Concentration µg/L (plasma)

    Recovery %

    Normetanephrine

    381

    98.2

    Metanephrine

    238

    95.2

    3-Methoxytyramine

    186

    104.1

  • Repeatability

     

    Analyte

    Concentration µg/L (plasma)

    CV %

    Normetanephrine

    38.5

    1.59

    Metanephrine

    29.1

    2.81

    3-Methoxytyramine

    19.3

    1.46

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..

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