HVA,VMA,5-HIAA KIT

HVA, VMA and 5HIAA

Laboratory measurement of vanillylmandelic acid (VMA), the product of epinephrine, norepinephrine metabolism, and homovanillic acid (HVA), a terminal metabolite of dopamine, and 5-Hydroxyindolacetic acid (5-HIAA) the end metabolite of serotonin is used to enhance clinical diagnosis and management of catecholamine-secreting neurochromaffin tumors such as neuroblastomas. Neuroblastoma usually emerges in children and accounts for 7–10% of childhood cancer; approximately 90% of these tumors produce catecholamines (CAs). Pheochromocytoma is a rare neuroendocrine tumor arising from the chromaffin cells of the adrenal medulla and characterized by producing excessive amounts of CAs and their metabolites.

  • Highlights

    • Simultaneously quantification of HVA, VMA and 5-HIAA

    • Dilute and shoot sample preparation procedure

    • Total run time 10 min.

  • parameters

    • Homovanillic acid
    • Vanillylmandelic acid
    • 5-Hydroxyindolacetic acid
  • Matrix

    • Urine

Sample Preparation


  • Step 1

    Pipette 50 µL of calibrator/control/urine sample into a sample via

  • Step 2

    Add 50 µL of IS solution, vortex for 5 sec

  • Step 3

    Add 400 µL of Reagent-1 addition to vortex for 5 sec. prior to injection

  • Extracted ion chromatogram of HVA, VMA and 5-HIAA

  • Retention Times of HVA, VMA and 5-HIAA

       Analytes

      R.T.     (minute)

        HVA

     4.76

       VMA

     3.34

    5-HIAA

     4.62

  • Linearity & Quantitation Limit (LOQ)

     

          

     

    URINE

    Compound 

    R2

    LOQ

    (µg/mL)

    HVA

    0.997

    1.77

    VMA

    0.998

    1.97

    5-HIAA

    0.999

    1.21

     

     

  • Calibration Curves of HVA, VMA and 5-HIAA

    Calibration Curve of HVA

    Calibration Curve of VMA

    Calibration Curve of 5-HIAA

  • Repeatability

            Compound

    Concentration µg/ml

    CV %

    HVA

    10.7

    3.54

    VMA

    10.4

    1.03

    5-HIAA

    17.3

    1.62

  • Recovery

            Compound

    Concentration µg/ml

    Recovery %

    HVA

    10.7

    103.9

    VMA

    10.4

    99.6

    5-HIAA

    17.3

    102.1

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..

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