HVA,VMA,5-HIAA KIT

HVA, VMA AND 5-HIAA IN URINE LC-MS/MS KIT

Laboratory measurement of vanillylmandelic acid (VMA), the end product of epinephrine, norepinephrine metabolism, and homovanillic acid (HVA), a terminal metabolite of dopamine, and 5-Hydroxyindolacetic acid (5-HIAA) the end metabolite of serotonin is used to enhance clinical diagnosis and management of catecholamine-secreting neurochromaffin tumors such as neuroblastomas. Neuroblastoma usually emerges in children and accounts for 7–10% of childhood cancer; approximately 90% of these tumors produce catecholamines (CAs). Pheochromocytoma is a rare neuroendocrine tumor arising from the chromaffin cells of the adrenal medulla and characterized by producing excessive amounts of CAs and their metabolites.
Jasem’s solution is offering simultaneously quantification of HVA, VMA and 5-HIAA using LC-MS/MS technology in 10 minutes subsequent to dilute and shoot sample preparation procedure.

  • Features

    • Analysis of HVA, VMA and 5-HIAA without derivatization.

    • Rapid and easy sample preparation; just dilution!

    • 10 minutes run.

  • parameters

    • Homovanillic acid
    • Vanillylmandelic acid
    • 5-Hydroxyindolacetic acid
  • Matrix

    • Urine

Sample Preparation


  • Step 1

    Pipet 50 µL of calibrator/control/urine sample into a sample vial and add 50 µL of IS solution, vortex for 5 seconds.

  • Step 2

    Add 400 µL of Reagent-1 addition to vortex for seconds prior to injection to the LC-MS/MS system.

  • Extracted ion chromatogram of HVA, VMA and 5-HIAA

  • Retention Times of HVA, VMA and 5-HIAA

       Analytes

      R.T.     (minute)

        HVA

     4.76

       VMA

     3.34

    5-HIAA

     4.62

  • Linearity & Quantitation Limit (LOQ)

     

          

     

    URINE

    Compound 

    R2

    LOQ

    (µg/mL)

    HVA

    0.997

    1.77

    VMA

    0.998

    1.97

    5-HIAA

    0.999

    1.21

     

     

  • Calibration Curves of HVA, VMA and 5-HIAA

    Calibration Curve of HVA

    Calibration Curve of VMA

    Calibration Curve of 5-HIAA

  • Repeatability

            Compound

    Concentration µg/ml in urine

    CV %

    HVA

    10.7

    3.54

    VMA

    10.4

    1.03

    5-HIAA

    17.3

    1.62

  • Recovery

            Compound

    Concentration µg/ml in urine

    Recovery %

    HVA

    10.7

    103.9

    VMA

    10.4

    99.6

    5-HIAA

    17.3

    102.1

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..

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The easiest way is to fill the contact form. Simply mail us to info@jasem.com.tr or give us a call +902165720200