Serotonin in Plasma

Serotonin LC-MS/MS Kit

Serotonin (5-hydroxytryptamine) is a monoamine neurotransmitter synthesized from the amino acid tryptophan. Most of total body serotonin is found in the gastrointestinal (GI) tract, where it modulates the functions of the bowel by controlling smooth muscle contraction. It also acts as a cardiovascular vasoconstriction mediator and in the brain as a neurotransmitter. Neuroendocrine neoplasms (NENs), which originate from GI tract enterochromaffin cells, may produce large amounts of serotonin.

  • Highlights

    • Total run time is 6 min.

    • Just a few pipetting steps for the sample treatment-protein precipitate and shoot approachno need for derivatization

    • Consuming small volume of patient’s sample

    • Long life span of HPLC column
  • Matrix

    • Plasma
  • Parameters

    • Serotonin

Sample Preparation


  • Step 1

    Pipette 150 µL of serum sample/working calibration standard into a glass centrifuge tube

  • Step 2

    Add 20 µL of internal standard working stock solution, vortex for 5 sec.

  • Step 3

    Add 250 µL of Reagent-1, additionally vortex for 15 sec. then centrifuge at 4000 rpm for 5 min.

  • Step 4

    Decant the clear supernatant into HPLC vial prior to injection

  • Extracted ion chromatogram of serotonin

  • Retention times of serotonin

                   Analyte

    Retention Times(min)

            Serotonin

                 3.57

  • Linearity & Limit of Quantitation

          

     

    PLASMA

    Analyte

    R2

    LOQ

    (µg/L)

    Serotonin

    0.998

    3.98

  • Recovery

    Analyte

    Concentration µg/L (plasma)

    Recovery %

    Serotonin

    225.0

    94.4

  • Repeatability

    Analyte

    Concentration µg/L (plasma)

    CV %

    Serotonin

    100.0

    0.39

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..

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