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Busulfan & Methotrexate in Serum/Plasma

Busulfan & Methotrexate in Serum/Plasma LC-MS/MS Analysis Kit

The efficacy and toxicity of anticancer drugs are related to parent drug and/or metabolite concentrations in body fluids and tissues. Therefore, monitoring of anticancer drugs in biological fluids and tissues is important during both pre-clinical and clinical development and often in routine clinical use.

  • Highlights

    Simultaneously quantification of Busulfan and Methotrexate.

    Total run time 5 min.

    Just a few pipetting steps for the sample treatment-protein precipitate and shoot approach-no need for derivatization.

    Consuming small volume of patient’s sample.

    Long life span of HPLC column.
  • Parameters

    Busulfan, Methotrexate.
  • Matrix

    Serum, plasma.

Sample Preparation

  • Step 1
    Pipette 100 µl serum sample/calibrators in a centrifuge tube.
  • Step 2
    Add 50 µL of IS solution, vortex for 5 sec.
  • Step 3
    Add 650 µl Reagent-1 to the sample and vortex 30 sec.
  • Step 4
    Centrifuge 3000 rpm for 5 min and decant the supernatant into a HPLC vial.
  • Chromatogram

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