Epilepsy is defined as seizure disorder induced by surge of electrical activity in the brain. Anticonvulsant or antiepileptic medications encompass a broad range of drugs that are primarily intended to control ongoing or anticipated future seizures as well as being beneficial in non-epileptic conditions such as psychiatric disorders and pain syndromes. Many antiepileptic drugs (AEDs) have a narrow therapeutic range thus relatively modest alterations of drug concentration in plasma can lead to loss response or toxic effects. Therefore, it is necessary to simultaneously determine AEDs including their active metabolite to improve treatments without undesirable side effects.