Clinical Toxicology in Urine LC-MS/MS Analysis Kit
Jasem Clinical Toxicology (CT) method introduces a procedure for detecting drugs of abuse such as benzodiazepines, antidepressants, atypical antipsychotics, antiepileptics, over-the-counter cold medicines as well as routinely used prescription drugs by follow up patients in urine samples by LC/MS-MS. Jasem method provides analysis of 116 drugs in urine with same sample preparation procedure. 116 drugs are analyzed using this method in 12 minutes with gradient elution. Jasem- Clinical Toxicology (CT) method facilitate the confirmatory of drugs implementing straightforward sample preparation (just protein precipitation) protocol.
LC-MS/MS is currently the best technique available for the correct quantification of Clinical Toxicological drugs.
According to customer needs, Jasem clinical toxicology kit is suitable to extend the drug list with new compounds.
Chromatogram
METHOD PERFORMANCE
| LOQ | LOQ | LOQ | Accuracy | Repeatability | ||
| Agilent 6460 | Agilent Ultivo | Agilent 6470 | Agilent 6470 | Agilent 6470 | ||
| (ug/L) | (ug/L) | (ug/L) | % | %RSD | ||
| 1 | 6-Acetylmorphine | 1,16 | 0,18 | 0,33 | 99 | 5,56 |
| 2 | 7-Aminoclonazepam | 1,26 | 0,25 | 0,34 | 109 | 5,74 |
| 3 | Alprazolam | 0,57 | 0,14 | 0,29 | 104 | 5,48 |
| 4 | Amisulpride | 0,53 | 0,01 | 0,3 | 96 | 5,26 |
| 5 | Amitriptyline | 0,91 | 0,1 | 0,44 | 100 | 5,31 |
| 6 | Amlodipine | – | 0,33 | 0,7 | 99 | 6,76 |
| 7 | Amphetamine | 1,16 | 0,87 | 0,36 | 107 | 7,79 |
| 8 | Atenolol | 0,71 | 0,09 | 0,28 | 98 | 4,88 |
| 9 | Atropine | 0,82 | 0,15 | 0,29 | 101 | 5,65 |
| 10 | Benzoylecgonine | 0,71 | 0,17 | 0,36 | 103 | 5,15 |
| 11 | Biperiden | 0,95 | 0,05 | 0,33 | 104 | 5,83 |
| 12 | Bromazepam | 1,47 | 0,49 | 0,46 | 104 | 5,85 |
| 13 | Buprenorphine | 1,53 | 0,78 | 4,02 | 109 | 6,46 |
| 14 | Carbamazepine | 0,93 | 0,04 | 0,4 | 102 | 5,45 |
| 15 | Chlordiazepoxide | 1,15 | 0,09 | 0,5 | 102 | 5,57 |
| 16 | Chlorpheniramine | 1 | 0,29 | 0,34 | 101 | 5,47 |
| 17 | Chlorpromazine | 1,1 | 0,01 | 0,32 | 103 | 5,6 |
| 18 | Citalopram + Escitalopram | 0,91 | 0,12 | 0,4 | 105 | 5,69 |
| 19 | Clobazam (Urbadan) | 0,44 | 0,05 | 0,35 | 103 | 5,8 |
| 20 | Clomipramine | 1,77 | 0,04 | 0,44 | 102 | 5,92 |
| 21 | Clonazepam | 1,37 | 0,22 | 0,79 | 106 | 6,02 |
The concentrations used in the accuracy and repeatability study included in the method performance; Pentobarbital 150 ug/L , Phenobarbital; 20 ug/L , Pantoprazole: 10 ug/L, Others;5 ug/L