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Clinical Toxicology in Urine

Clinical Toxicology in Urine LC-MS/MS Analysis Kit

Jasem Clinical Toxicology (CT) method introduces a procedure for detecting drugs of abuse such as benzodiazepines, antidepressants, atypical antipsychotics, antiepileptics, over-the-counter cold medicines as well as routinely used prescription drugs by follow up patients in urine samples by LC/MS-MS. Jasem method provides analysis of 116 drugs in urine with same sample preparation procedure. 116 drugs are analyzed using this method in 12 minutes with gradient elution. Jasem- Clinical Toxicology (CT) method facilitate the confirmatory of drugs implementing straightforward sample preparation (just protein precipitation) protocol.

LC-MS/MS is currently the best technique available for the correct quantification of Clinical Toxicological drugs.

According to customer needs, Jasem clinical toxicology kit is suitable to extend the drug list with new compounds.

Highlights

Quick and simple sample preparation steps without SPE or evaporation

Dilute and shoot for urine samples

Quantitative determination of 116 drugs in a single injection

Simultaneous analysis of different drug groups-total run time is 12 minutes

Long lifetime HPLC column

Method allows to extend the drug list

Parameters

at the bottom of the page

Matrix

Urine

Sample Preparation


  • Step 1
    Take 200 μl urine sample into a centrifuge tube
  • Step 2
    Add 800 μl of Reagent-1 and vortex for 15 seconds.
  • Step 3
    If necessary filter out from 0.45 micron nylon or decant directly into an HPLC vial

Chromatogram

METHOD PERFORMANCE

    LOQ  LOQ  LOQ  Accuracy Repeatability
Agilent 6460  Agilent Ultivo  Agilent 6470  Agilent 6470 Agilent 6470
    (ug/L) (ug/L) (ug/L) % %RSD
1 6-Acetylmorphine 1,16 0,18 0,33 99 5,56
2 7-Aminoclonazepam 1,26 0,25 0,34 109 5,74
3 Alprazolam 0,57 0,14 0,29 104 5,48
4 Amisulpride 0,53 0,01 0,3 96 5,26
5 Amitriptyline 0,91 0,1 0,44 100 5,31
6 Amlodipine 0,33 0,7 99 6,76
7 Amphetamine 1,16 0,87 0,36 107 7,79
8 Atenolol 0,71 0,09 0,28 98 4,88
9 Atropine 0,82 0,15 0,29 101 5,65
10 Benzoylecgonine 0,71 0,17 0,36 103 5,15
11 Biperiden 0,95 0,05 0,33 104 5,83
12 Bromazepam 1,47 0,49 0,46 104 5,85
13 Buprenorphine 1,53 0,78 4,02 109 6,46
14 Carbamazepine 0,93 0,04 0,4 102 5,45
15 Chlordiazepoxide 1,15 0,09 0,5 102 5,57
16 Chlorpheniramine 1 0,29 0,34 101 5,47
17 Chlorpromazine 1,1 0,01 0,32 103 5,6
18 Citalopram + Escitalopram 0,91 0,12 0,4 105 5,69
19 Clobazam (Urbadan) 0,44 0,05 0,35 103 5,8
20 Clomipramine 1,77 0,04 0,44 102 5,92
21 Clonazepam 1,37 0,22 0,79 106 6,02

The concentrations used in the accuracy and repeatability study included in the method performance; Pentobarbital 150 ug/L , Phenobarbital; 20 ug/L , Pantoprazole: 10 ug/L, Others;5 ug/L

PARAMETERS

1

6-Acetylmorphine

40

Etodolac

79

Nifedipine

2

7-Aminoclonazepam

41

Famotidine

80

Nitrazepam

3

Alprazolam

42

Fentanyl

81

Norbuprenorphine

4

Amisulpride

43

Fluconazole

82

Nordiazepam

5

Amitriptyline

44

Flunitrazepam

83

Nortriptyline

6

Amlodipine

45

Fluoxetine

84

Olanzapine

7

Amphetamine

46

Flurazepam

85

Opipramol

8

Atenolol

47

Fluvoxamine

86

Ornidazole

9

Atropine

48

Gabapentin

87

Oxazepam

10

Benzoylecgonine

49

Glibenclamide

88

Oxcarbazepine

11

Biperiden

50

Haloperidol

89

Oxycodone

12

Bromazepam

51

Hydroxyzine

90

Paliperidone

13

Buprenorphine

52

Imipramine

91

Pantoprazole

14

Carbamazepine

53

Ketamine

92

Paracetamol

15

Chlordiazepoxide

54

Lansoprazole

93

Paroxetine

16

Chlorpheniramine

55

Levetiracetam

94

Pentobarbital

17

Chlorpromazine

56

Lidocaine

95

Pentoxifylline

18

Citalopram

57

Loperamide

96

Pethidine

19

Clobazam

58

Lorazepam

97

Pheniramine

20

Clomipramine

59

MBDB

98

Phenobarbital

21

Clonazepam

60

MDA

99

Phenytoin

22

Clozapine

61

MDEA

100

Prilocaine

23

Cocaethylene

62

MDMA

101

Propafenone

24

Cocaine

63

Medazepam

102

Propranolol

25

Codeine

64

Memantine

103

Propyphenazone

26

Desipramine

65

Metformin

104

Pseudoephedrine + Ephedrine

27

Desmethylclomipramine

66

Methadone

105

Quetiapine

28

Desmethylolanzapine

67

Methamphetamine

106

Ranitidine

29

Dextromethorphan

68

Methylecgonine

107

Risperidone

30

Diazepam

69

Metoclopramide

108

Sertraline

31

Diclofenac

70

Metoprolol

109

THC  delta9-Tetrahydrocannabinol

32

Digoxin

71

Metronidazole

110

THC-COOH

33

Dihydrocodeine

72

Mianserin

111

THC-OH 11-OH-tetrahydrocannabinol

34

Diltiazem

73

Midazolam

112

Thiopental

35

Diphenhydramine

74

Mirtazepine

113

Thioridazine

36

Doxepin

75

Moclobemide

114

Tramadol

37

Doxylamine

76

Morphine

115

Trimipramine

38

EDDP

77

Naproxen

116

Venlafaxine

39

Ethylmorphine

78

N-desmethylclozapine

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