Skip to main content
TOTAL HOMOCYSTEINE

Total Homocysteine in Serum/Plasma LC-MS/MS Analysis Kit

Homocysteine is a non-essential sulfur containing amino acid closely linked to the essential amino acid methionine. Homocysteine is rapidly oxidized in plasma into homocystine and cysteine-homocysteine disulfide. Total plasma homocysteine is the sum of homocysteine and its oxidized forms. The determination of total homocysteine plays an indicator role in diagnosis and therapy of folate, vitamin B12 and vitamin B6 deficiencies. In addition, several observational studies have confirmed that increased total homocysteine is an independent predictor of cardiovascular risk.

  • Highlights

    Simply a reduction step followed by precipitation. No need for precolumn derivatisation.

    Total run time is 5 min.

    Safeguarded by its own internal standard.

    Small volume of patient’s sample is required.

    Long life span of HPLC column.

  • Parameters

    Homocysteine

  • Matrix

    Serum/Plasma

Sample Preparation

  • Step 1
    Pipette 50 µL of serum/plasma or quality control sample into a glass centrifuge tube and add 50 µL of internal standard.
  • Step 2
    Vortex for 5 sec. and add 100 µL of reducing solution, again vortex for 15 sec. Then, incubate in a water bath at 60oC for 15 min.
  • Step 3
    Add 650 µL Reagent-2 and vortex for 5 seconds then centrifuge at 3000 rpm for 5 min.
  • Step 4
    Decant the clear supernatant into HPLC vial prior to injection.

  • Chromatogram

  • METHOD PERFORMANCE

    Analyte

    LOQ (mg/L)   6460 QQQ

    Accuracy % 6460 QQQ

    Repeatabilty % CV 6460 QQQ

    Linearity R2 6460 QQQ

    Homocysteine

    0,043

    102,0

    0,35

    0,999
ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..
PRODUCTS
COMPANY
Contact Us

The easiest way is to fill the contact form. Simply mail us to info@jasem.com.tr or give us a call +902165720200