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HVA-VMA-5HIAA in Urine

HVA-VMA-5HIAA in Urine LC-MS/MS Analysis Kit

Laboratory measurement of vanillylmandelic acid (VMA), the product of epinephrine, norepinephrine metabolism, and homovanillic acid (HVA), a terminal metabolite of dopamine, and 5-Hydroxyindolacetic acid (5-HIAA) the end metabolite of serotonin is used to enhance clinical diagnosis and management of catecholamine-secreting neurochromaffin tumors such as neuroblastomas. Neuroblastoma usually emerges in children and accounts for 7–10% of childhood cancer; approximately 90% of these tumors produce catecholamines (CAs). Pheochromocytoma is a rare neuroendocrine tumor arising from the chromaffin cells of the adrenal medulla and characterized by producing excessive amounts of CAs and their metabolites.

  • Highlights

    Simultaneously quantification of HVA, VMA and 5-HIAA

    Dilute and shoot sample preparation procedure

    Total run time 10 min.
  • Parameters

    Homovanillic acid

    Vanillylmandelic acid

    5-Hydroxyindolacetic acid
  • Matrix


Sample Preparation

  • Step 1
    Pipette 50 µL of calibrator/control/urine sample into a sample via
  • Step 2
    Add 50 µL of IS solution, vortex for 5 sec
  • Step 3
    Add 400 µL of Reagent-1 addition to vortex for 5 sec. prior to injection
  • Chromatogram


    Analyte LOQ (mg/L)   6460 QQQ LOQ (mg/L)   Ultivo QQQ LOQ (mg/L)   6470 QQQ Accuracy % 6470 QQQ Repeatabilty % CV 6470 Linearity R2 6470 QQQ
    Homovanillic Acid 1,769 0,622 2,75 117,41 3,23 0,997
    Vanillylmandelic Acid 1,972 0,025 3,99 107,74 3,9 0,998
    5-Hydroxyindolacetic Acid 1,212 0,037 1,97 81,4 6,45 0,999
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