HPLC KIT FOR PKU

PKU HPLC ANALYSIS KIT IN PLASMA & DBS SAMPLES

Phenylketonuria, also called PKU, is a rare inherited disorder that causes an amino acid called
phenylalanine to build up in the body. PKU is caused by a defect in the gene that helps create the
enzyme needed to break down phenylalanine.
Without the enzyme necessary to process phenylalanine, a dangerous buildup can develop when a
person with PKU eats foods that contain protein or eats aspartame, an artificial sweetener. This can
eventually lead to serious health problems.
For the rest of their lives, people with PKU — babies, children and adults — need to follow a diet
that limits phenylalanine, which is found mostly in foods that contain protein.
Babies are screened for PKU soon after birth. Recognizing PKU right away can help prevent major
health problems.
It can be quantitatively determined by the Jasem PKU HPLC Analysis Kit.

  • Features

    • Quick and simple sample preparation steps, no need to use SPE for clean up

    • 11.0 minutes analysis time.

    • Quick and simple sample preparation steps-just protein precipitation for plasma

    • Quick and simple sample preparation steps-just extraction for dbs

    • Isocratic mobile phase gradient
  • PARAMETERS

    • Phenylalanine
    • Tyrosine
    • Tryptophan
  • Matrix

    • Plasma, Dried Blood Spot

Sample preparation for plasma samples


  • Step 1

    Pipet 50 μl of plasma sample into a clean centrifuge tube and add 300 μl of Reagent-1 (protein precipitation) Mix by vortex for 5 seconds and incubate for 15 minutes at 4 °C

  • Step 2

    Then, centrifuge at 5000 rpm for 5 minutes.Decant the supernatant into HPLC vial and inject to the HPLC system

Sample preparation for DBS samples


  • Step 1

    Punch 3.0 mm of specimen from DBS

  • Step 2

    Add 200 μl of Reagent-1 mixture for extraction and shake gently 10 minutes then transfer the supernatant to a vial and inject to HPLC system

  • Example chromatogram for plasma sample

  • METHOD PERFORMANCE

    (PLASMA)

    ( DBS )

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..

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