Multi-Antibiotics In Honey LC-MS/MS Analysis Kit
Antibiotics are widely used in veterinary medicine for prevention of disease in food producing animals. Excessive or unconscious usage of antibiotics might result residue in food such as milk, meat, honey and others. These residues can cause toxicity, allergic reactions. In additions low antibiotics residues in food products, consumed for long time and lead to the spread of drug-resistant bacteria. On the other hand, milk goes through production, processing and circulation until reaching consumers. In order to ensure consumers’ safety and to prevent any harmful health effects, WHO and EU established the MRL for antibiotics. LC-MS/MS has become the method of choice for analysis antibiotics residues due to do its high sensitivity, selectivity, robustness and cost effective.
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Highlights
Analysis of multi-antibiotics in one method
No need SPE or immunoaffinity column for extraction
Quick and simple sample preparation -
Parameters
at the bottom of the page -
Matrix
Honey
Sample Preparation
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Step 15 g of honey sample is weighed into the plastic centrifuge tube.
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Step 2Add 200 µl ISTD solution, 20 ml Reagent-1 on the sample and shake for 15 minutes.
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Step 3Centrifuge at 3000 rpm for 10 min.
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Step 4Filter the clear supernatant from 0.45 µm PTFE filter into a HPLC vial.
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Chromatogram
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METHOD PERFORMANCE
Analytes
LOQ (µg/L) ULTIVO QQQ
Accuracy (%) ULTIVO QQQ
Repeatability (%RSD) ULTIVO QQQ
Linearity (R2) ULTIVO QQQ
Albendazole
0.89
92
0.45
0.999
Chloramphenicol
2.45
92
1.39
0.995
Chlortetracycline
2.79
98
1.28
0.993
Ciprofloxacin
3.10
80
2.65
0.992
Dihydrostreptomycin
0.42
70
0.20
0.998
Doxycycline
3.08
83
1.77
0.996
Enrofloxacin
1.48
90
0.96
0.997
Erythromycin
1.78
78
0.97
0.998
Fenbendazole
0.87
84
0.43
0.996
Flubendazole
0.94
100
0.48
0.994
Furazolidon
1.24
81
0.64
0.992
Levamisole
0.63
87
0.33
0.995
marbofloxacin
2.49
85
1.25
0.996
Mebendazole
1.36
95
0.68
0.997
Oxolinic acid
0.77
93
0.42
0.998
Oxytetracycline
3.11
100
1.71
0.996
Penicillin G
2.50
94
1.85
0.992
Streptomycin
2.10
104
0.84
0.996
Sulfachloropyridazine
1.64
88
0.84
0.996
Sulfadiazine (Silvadene)
0.59
85
0.30
0.999
Sulfadimethoxine
0.86
90
0.46
0.999
Sulfadimidine(Sulfamethazine)
1.56
85
0.78
0.998
Sulfadoxine
0.86
91
0.46
0.993
Sulfamerazine
1.18
89
0.55
0.995
Sulfamethoxazole
1.22
91
0.60
0.997
Sulfapyridine
2.43
83
1.30
0.996
Sulfaquinoxaline
0.92
96
0.48
0.999
Sulfathiazole
2.14
81
1.19
0.991
Tetracycline
3.22
100
2.08
0.993
Thiabendazole
0.71
91
0.40
0.996
Tylosin
2.17
76
1.31
0.992
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Albendazole, Chloramphenicol, Chlortetracycline, Ciprofloxacin, Dihydrostreptomycin, Doxycycline, Enrofloxacin, Erythromycin, Fenbendazole, Flubendazole, Furazolidon, Levamisole, marbofloxacin, Mebendazole, Oxolinic acid, Oxolinic acid, Oxytetracycline, Penicillin G, Sulfadoxine, Sulfamerazine, Sulfamethoxazole, Sulfapyridine, Sulfaquinoxaline, Sulfathiazole, Sulfaquinoxaline, Sulfathiazole, Sulfachloropyridazine, Sulfadiazine (Silvadene), Sulfadimethoxine, Sulfadimidine (Sulfamethazine), Streptomycin, Tetracycline, Thiabendazole, Tylosin.