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Multi-Antibiotics In Honey

Multi-Antibiotics In Honey LC-MS/MS Analysis Kit

Antibiotics are widely used in veterinary medicine for prevention of disease in food producing animals. Excessive or unconscious usage of antibiotics might result residue in food such as milk, meat, honey and others. These residues can cause toxicity, allergic reactions. In additions low antibiotics residues in food products, consumed for long time and lead to the spread of drug-resistant bacteria. On the other hand, milk goes through production, processing and circulation until reaching consumers.  In order to ensure consumers’ safety and to prevent any harmful health effects, WHO and EU established the MRL for antibiotics. LC-MS/MS has become the method of choice for analysis antibiotics residues due to do its high sensitivity, selectivity, robustness and cost effective.

  • Highlights

    Analysis of multi-antibiotics in one method

    No need SPE or immunoaffinity column for extraction

    Quick and simple sample preparation
  • Parameters

    at the bottom of the page
  • Matrix

    Honey

Sample Preparation


  • Step 1
    5 g of honey sample is weighed into the plastic centrifuge tube.
  • Step 2
    Add 200 µl ISTD solution, 20 ml Reagent-1 on the sample and shake for 15 minutes.
  • Step 3
    Centrifuge at 3000 rpm for 10 min.
  • Step 4
    Filter the clear supernatant from 0.45 µm PTFE filter into a HPLC vial.
  • Chromatogram

  • METHOD PERFORMANCE

    Analytes

    LOQ (µg/L) ULTIVO QQQ

    Accuracy (%) ULTIVO QQQ

    Repeatability (%RSD) ULTIVO QQQ

    Linearity (R2) ULTIVO QQQ

    Albendazole

    0.89

    92

    0.45

    0.999

    Chloramphenicol

    2.45

    92

    1.39

    0.995

    Chlortetracycline

    2.79

    98

    1.28

    0.993

    Ciprofloxacin

    3.10

    80

    2.65

    0.992

    Dihydrostreptomycin

    0.42

    70

    0.20

    0.998

    Doxycycline

    3.08

    83

    1.77

    0.996

    Enrofloxacin

    1.48

    90

    0.96

    0.997

    Erythromycin

    1.78

    78

    0.97

    0.998

    Fenbendazole

    0.87

    84

    0.43

    0.996

    Flubendazole

    0.94

    100

    0.48

    0.994

    Furazolidon

    1.24

    81

    0.64

    0.992

    Levamisole

    0.63

    87

    0.33

    0.995

    marbofloxacin

    2.49

    85

    1.25

    0.996

    Mebendazole

    1.36

    95

    0.68

    0.997

    Oxolinic acid

    0.77

    93

    0.42

    0.998

    Oxytetracycline

    3.11

    100

    1.71

    0.996

    Penicillin G

    2.50

    94

    1.85

    0.992

    Streptomycin

    2.10

    104

    0.84

    0.996

    Sulfachloropyridazine

    1.64

    88

    0.84

    0.996

    Sulfadiazine (Silvadene)

    0.59

    85

    0.30

    0.999

    Sulfadimethoxine

    0.86

    90

    0.46

    0.999

    Sulfadimidine(Sulfamethazine)

    1.56

    85

    0.78

    0.998

    Sulfadoxine

    0.86

    91

    0.46

    0.993

    Sulfamerazine

    1.18

    89

    0.55

    0.995

    Sulfamethoxazole

    1.22

    91

    0.60

    0.997

    Sulfapyridine

    2.43

    83

    1.30

    0.996

    Sulfaquinoxaline

    0.92

    96

    0.48

    0.999

    Sulfathiazole

    2.14

    81

    1.19

    0.991

    Tetracycline

    3.22

    100

    2.08

    0.993

    Thiabendazole

    0.71

    91

    0.40

    0.996

    Tylosin

    2.17

    76

    1.31

    0.992

  • Albendazole, Chloramphenicol, Chlortetracycline, Ciprofloxacin, Dihydrostreptomycin, Doxycycline, Enrofloxacin, Erythromycin, Fenbendazole, Flubendazole, Furazolidon, Levamisole, marbofloxacin, Mebendazole, Oxolinic acid, Oxolinic acid, Oxytetracycline, Penicillin G, Sulfadoxine, Sulfamerazine, Sulfamethoxazole, Sulfapyridine, Sulfaquinoxaline, Sulfathiazole, Sulfaquinoxaline, Sulfathiazole, Sulfachloropyridazine, Sulfadiazine (Silvadene), Sulfadimethoxine, Sulfadimidine (Sulfamethazine), Streptomycin, Tetracycline, Thiabendazole, Tylosin.
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