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MULTI-MYCOTOXINS IN BABY FOOD LC-MS/MS ANALYSIS KIT

Multi-Mycotoxins in Baby Food

Mycotoxins are secondary metabolites produced by molds, without biochemical significance in fungal growth and development. Although they have existed since ancient times, the term mycotoxins appeared in 1960 when 100 000 turkey poults died due to the ingestion of peanut contaminated with secondary metabolites from Aspergillus flavus.

Mycotoxins are compounds with various chemical structures and, therefore, various biological effects in animals and humans such as toxic, carcinogenic, estrogenic, mutagenic, teratogenic, and immunotoxic effects.

In Europe, Commission Regulation (EC) No 1881/2006 and its amendments set maximum residue levels (MRLs) for 13 mycotoxins.

Jasem multi-mycotoxin method analyzes 10 mycotoxins in one injection and HT-2 and DON are analyzed in second injection with same sample, same column and same instrument parameters except HPLC program.

Highlights

No need for immunoaffinity column extraction.
Very easy sample preparation.
No source contamination.

Parameters

AF B1, AF B2, AF G1, AF G2, FB1, FB2, OTA, DON, ZON, HT2, T2, STE

Matrix

Baby food (based cereal)

Sample Preparation

  • Step 1
    Weigh 2.0 g of fine blended baby food matrix (cereal or milk based) into a 50 ml polypropylene falcon.
  • Step 2
    Add 10 ml Reagent 1 onto sample and shake for 15 minutes using multiple-shaker.
  • Step 3
    Pipet 1.0 ml from the 10 ml milk powder solution into a glass centrifuge tube
  • Step 4
    Filter the clear supernatant from 0.45 micron nylon filter into a HPLC vial.

Chromatogram

METHOD PERFORMANCE

LOQ

AFB1

AFB2

AFG1

AFG2

DON

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

0,05

0,20

0,27

0,28

21,93

5,46

5,50

4,74

0,10

0,13

1,09

1,10

R2

0,998

0,997

0,997

0,998

0,997

0,998

0,998

0,997

0,997

0,999

0,998

0,997

Repeatability

Level 1

AFB1

AFB2

AFG1

AFG2

DON

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

0,3125

0,3125

0,3125

0,3125

31,25

31,25

9,375

31,25

0,3125

0,3125

3,125

3,125

RSD (%)

 

4,61

4,06

4,84

7,60

2,06

5,00

2,94

8,69

9,84

5,94

10,03

5,41

Level 2

 

 

 

 

 

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

0,625

0,625

0,625

0,625

62,5

62,5

18,75

62,5

0,625

0,625

6,25

6,25

RSD (%)

 

7,27

4,9

3,41

6,71

5,52

3,27

2,96

8,00

3,65

3,53

4,78

3,93

Level 3

 

 

 

 

 

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

1,25

1,25

1,25

1,25

125

125

37,5

125

1,25

1,25

12,5

12,5

RSD (%)

 

1,17

2,05

3,33

3,35

4,09

3,3

2,38

2,53

3,9

2,56

4,85

1,37

Level 4

 

 

 

 

 

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

1,875

1,875

1,875

1,875

187,5

187,5

56,25

187,5

1,875

1,875

18,75

18,75

RSD (%)

 

2,32

2,86

2,17

5,54

2,00

3,8

2,17

2,07

3,11

1,83

5,51

4,83

reproducibility

Level 1

AFB1

AFB2

AFG1

AFG2

DON

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

0,3125

0,3125

0,3125

0,3125

31,25

31,25

9,375

31,25

0,3125

0,3125

3,125

3,125

RSD (%)

 

4,87

6,98

4,59

5,23

3,49

5,08

3,21

3,12

10,54

7,09

5,83

7,48

Level 2

 

 

 

 

 

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

0,625

0,625

0,625

0,625

62,5

62,5

18,75

62,5

0,625

0,625

6,25

6,25

RSD (%)

 

7,97

4,61

6,75

14,61

4,43

5,53

2,81

8,23

10,06

10,1

9,48

6,75

Level 3

 

 

 

 

 

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

1,25

1,25

1,25

1,25

125

125

37,5

125

1,25

1,25

12,5

12,5

RSD (%)

 

6,55

4,81

5,44

12,72

5,80

6,50

4,53

4,93

7,13

5,40

5,68

3,96

Level 4

 

 

 

 

 

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

1,875

1,875

1,875

1,875

187,5

187,5

56,25

187,5

1,875

1,875

18,75

18,75

RSD (%)

 

5,28

4,14

4,40

11,54

3,21

5,66

4,99

4,34

7,02

9,40

6,73

4,20

recovery

Level 1

AFB1

AFB2

AFG1

AFG2

DON

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

0,3125

0,3125

0,3125

0,3125

31,25

31,25

9,375

31,25

0,3125

0,3125

3,125

3,125

Recovery (%)

 

105,73

110,35

110,80

111,46

115,43

82,55

94,20

105,81

107,46

102,77

106,10

105,95

Level 2

 

 

 

 

 

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

0,625

0,625

0,625

0,625

62,5

62,5

18,75

62,5

0,625

0,625

6,25

6,25

Recovey (%)

 

98,11

102,00

102,34

96,30

108,67

82,53

92,97

97,94

91,17

100,27

96,47

107,61

Level 3

 

 

 

 

 

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

1,25

1,25

1,25

1,25

125

125

37,5

125

1,25

1,25

12,5

12,5

Recovery  (%)

 

101,24

101,47

95,55

92,43

101,87

89,90

94,60

98,94

94,52

96,04

96,05

110,65

Level 4

 

 

 

 

 

FB1

FB2

HT2

OTA

STE

T2

ZON

Conc. (µg/kg)

1,875

1,875

1,875

1,875

187,5

187,5

56,25

187,5

1,875

1,875

18,75

18,75

Recovery (%)

 

103,10

105,32

101,24

99,08

99,20

93,70

100,27

108,37

98,12

99,08

99,62

112,60
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