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ORGANIC ACID IN SERUM

Organic Acid in Serum LC-MS/MS Analysis Kit

Human urine is a complex biofluid of diverse small polar metabolites such as organic acids. Organic acidurias are inborn errors of metabolism given rise to by defections of inherited enzymes which lead to accumulation excess amounts of normal organic acids in human urine. For this reason qualitative and quantitative analyses of urinary organic acids are prominent diagnostic tools for the interpretation of metabolite profile that reflects inherited metabolic disorders.

“Jasem-Organic Acids” method overcomes abovementioned drawbacks by using liquid chromatography tandem mass spectrometry (LC-MS/MS). Jasem method ensures quantification of 54 organic acids by absence of derivatization simplified sample preperation to diluting. Jasem offers two panels achieving sufficient chromatographic separation for crucial isomers by LC-MS/MS.

Highlights

12 minutes run for each panel (Total analysis time 24 minutes-Panel 1&2).

Analysis of 54 organic acids without derivatization.

Quick and easy sample preparation; just dilution!

Successful chromatographic separation of isomers.

Consuming small volume of patient’s sample.

Parameters

at the bottom of the page

Matrix

Serum

Sample Preparation


  • Step 1
    Dilute serum sample 10 times using Reagent-1
  • Step 2
    Transfer 100 µl of diluted urine sample to a vial
  • Step 3
    Add 50 µl of IS mix and 850 µl of Reagent-1 into the sample and inject to LC-MS/MS system

Chromatogram

METHOD PERFORMANCE

Analytes

LOQ

(ppm)

Ultivo

Linearity

(R2)

Ultivo

Recovery

Recovery %

Ultivo

Repeatability

% RSD

Ultivo

2-Hydroxy isovaleric acid

0,24

0,999

101,6

6,07

2-Metyl-citrate

0,55

0,997

118,92

2,17

2-OH-3-Methyl pentanoic acid

3,56

0,999

100,2

9,11

2-OH-Butyric acid

2,33

0,997

90,71

7,97

2-OH-glutaric acid

3,87

0,998

85,2

3,47

2-OH-isocaproic acid

0,50

0,999

100,8

12,29

2-OH-Phenyl acetic acid

0,07

0,999

94,05

4,92

2-Oxoadipic acid

0,18

0,999

106,25

13,01

3-Hydroxyisovaleric acid

0,67

0,999

94,5

7,96

3-Hydroxypropanoic acid

1,37

0,994

51,0

8,39

3-Methyl -2-oxovaleric acid

0,99

0,995

89,9

3,96

3-Methyl crotonyl glycine

0,33

0,999

103,5

7,76

3-Methylglutaconic acid

0,21

0,999

96,02

2,98

3-Methylglutaric acid

0,26

0,999

100,5

8,34

3-OH-2methyl butanoic acid

0,23

0,999

103,51

8,63

3-OH-3-Methyl-glutaric acid

0,79

0,999

98,78

4,08

3-OH-butyric acid

4,01

0,998

99,33

3,73

3-OH-glutaric acid

0,33

0,999

118,0

3,25

3-OH-Isobutyrate

2,25

0,998

83,32

4,35

3-OH-pentanoic acid

0,15

0,999

98,4

8,97

3-Phenyl lactic acid

0,09

0,998

91,14

3,92

4-Methyl-2-oxovaleric acid

0,64

0,999

94,1

10,89

4-OH-Phenyl-Acetic acid

3,00

0,999

92,06

11,87

4-OH-phenylpyruvic acid

3,74

0,994

74,0

1,54

Adipic acid

0,78

0,998

84,0

9,36

Alpha-ketoglutaric acid

3,60

0,998

109,0

6,21

Citric acid

3,56

0,999

98,3

1,81

Ethylmalonic acid

0,34

0,999

94,5

8,88

Fumaric acid

1,50

0,992

60,69

6,47

Glutaconic acid

2,88

0,999

101,7

13,00

Glutaric acid

2,47

0,999

103,9

3,56

Glycolic acid

3,25

0,998

122,61

5,51

hexanoyl glycine

0,06

0,999

96,77

4,17

Homogentisic acid

0,05

0,996

88,64

6,87

Lactic acid

1,38

0,997

83,2

6,73  

Malic acid

4,97

0,995

74,36

2,14

Malonic acid

0,37

0,999

94,84

8,36

Methylmalonic acid

0,60

0,999

103,2

0,57

N-(3-phenyl propionyl) glycine

0,03

0,998

79,61

2,64

N-Acetylaspartic acid

0,26

0,999

75,66

6,22

N-Acetyltyrosine

0,08

0,999

96,83

5,95

N-isovaleryl glycine

0,18

0,999

85,58

7,27

Orotic acid

0,64

0,992

61,0

12,74

Oxoproline

2,55

0,997

85,47

4,32

Phenyl pyrivuc acid

0,14

0,999

104,5

7,43

P-OH-phenyl lactic acid

0,17

0,997

87,79

8,46

Propionyl glycine

0,20

0,993

71,02

9,06

Pyruvic acid

3,27

0,938

102,0

13,25

Sebacic acid

0,11

0,999

114,26

4,25

Suberic acid

0,13

0,999

114,56

4,77

Suberylglycine

0,18

0,999

93,12

1,82

Succinic acid

5,85

0,999

92,6

6,88

Succunylacetone

0,07

0,999

96,25

3,67

Tiglyl glycine

0,12

0,999

99,3

6,94

2-Methyl citrate, 2-OH-Phenylacetic acid, 2-OH-Butyric acid, 2-Oxoadipic acid, 3-Methylglutaconic acid, 3-OH-2-Methylbutanoic acid, 3-OH-3-Methylglutaric acid, 3-OH-Butyric acid, 3-OH-Isobutyrate, 3-Phenyllactic acid, 4-OH-Phenylacetic acid, Fumaric acid, Glycolic acid, Hexanoylglycine, Homogentisic acid, Malic acid, Malonic acid, N-(3-phenyl propionyl) glycine, N-Acetylaspartic acid, N-Acetyltyrosine, N-isovaleryl glycine, Oxoproline, 4-OH-Phenyl lactic acid, Propionylglycine, Sebacic acid, Suberic acid, Suberylglycine, Succinylacetone, 2-OH-Glutaric acid, 2-OH-3-Methylpentanoic acid, 2-OH-Isocaproic acid, 2-OH-Isovaleric acid, 3-OH-Glutaric acid, 3-OH-Propanoic acid, 3-Methylcrotonyl glycine, 3-Methylglutaric acid, 3-Methyl-2-oxovaleric acid, 3-OH-Isovaleric acid, 3-OH-pentanoic acid, 4-Methyl-2-oxovaleric acid, 4-OH-Phenylpyruvic acid, Adipic acid, Alpha-Ketoglutaric acid, Citric acid, Ethylmalonic acid, Glutaconic acid, Glutaric acid, Lactic acid, Methylmalonic acid, Orotic acid, Phenylpyruvic acid, Pyruvic acid, Succinic acid, Tiglylglycine
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