Organic Acid in Serum LC-MS/MS Analysis Kit
Human urine is a complex biofluid of diverse small polar metabolites such as organic acids. Organic acidurias are inborn errors of metabolism given rise to by defections of inherited enzymes which lead to accumulation excess amounts of normal organic acids in human urine. For this reason qualitative and quantitative analyses of urinary organic acids are prominent diagnostic tools for the interpretation of metabolite profile that reflects inherited metabolic disorders.
“Jasem-Organic Acids” method overcomes abovementioned drawbacks by using liquid chromatography tandem mass spectrometry (LC-MS/MS). Jasem method ensures quantification of 54 organic acids by absence of derivatization simplified sample preperation to diluting. Jasem offers two panels achieving sufficient chromatographic separation for crucial isomers by LC-MS/MS.
Chromatogram
METHOD PERFORMANCE
| Analytes | LOQ (ppm) Ultivo | Linearity (R2) Ultivo | Recovery Recovery % Ultivo | Repeatability % RSD Ultivo |
| 2-Hydroxy isovaleric acid | 0,24 | 0,999 | 101,6 | 6,07 |
| 2-Metyl-citrate | 0,55 | 0,997 | 118,92 | 2,17 |
| 2-OH-3-Methyl pentanoic acid | 3,56 | 0,999 | 100,2 | 9,11 |
| 2-OH-Butyric acid | 2,33 | 0,997 | 90,71 | 7,97 |
| 2-OH-glutaric acid | 3,87 | 0,998 | 85,2 | 3,47 |
| 2-OH-isocaproic acid | 0,50 | 0,999 | 100,8 | 12,29 |
| 2-OH-Phenyl acetic acid | 0,07 | 0,999 | 94,05 | 4,92 |
| 2-Oxoadipic acid | 0,18 | 0,999 | 106,25 | 13,01 |
| 3-Hydroxyisovaleric acid | 0,67 | 0,999 | 94,5 | 7,96 |
| 3-Hydroxypropanoic acid | 1,37 | 0,994 | 51,0 | 8,39 |
| 3-Methyl -2-oxovaleric acid | 0,99 | 0,995 | 89,9 | 3,96 |
| 3-Methyl crotonyl glycine | 0,33 | 0,999 | 103,5 | 7,76 |
| 3-Methylglutaconic acid | 0,21 | 0,999 | 96,02 | 2,98 |
| 3-Methylglutaric acid | 0,26 | 0,999 | 100,5 | 8,34 |
| 3-OH-2methyl butanoic acid | 0,23 | 0,999 | 103,51 | 8,63 |
| 3-OH-3-Methyl-glutaric acid | 0,79 | 0,999 | 98,78 | 4,08 |
| 3-OH-butyric acid | 4,01 | 0,998 | 99,33 | 3,73 |
| 3-OH-glutaric acid | 0,33 | 0,999 | 118,0 | 3,25 |
| 3-OH-Isobutyrate | 2,25 | 0,998 | 83,32 | 4,35 |
| 3-OH-pentanoic acid | 0,15 | 0,999 | 98,4 | 8,97 |
| 3-Phenyl lactic acid | 0,09 | 0,998 | 91,14 | 3,92 |
| 4-Methyl-2-oxovaleric acid | 0,64 | 0,999 | 94,1 | 10,89 |
| 4-OH-Phenyl-Acetic acid | 3,00 | 0,999 | 92,06 | 11,87 |
| 4-OH-phenylpyruvic acid | 3,74 | 0,994 | 74,0 | 1,54 |
| Adipic acid | 0,78 | 0,998 | 84,0 | 9,36 |
| Alpha-ketoglutaric acid | 3,60 | 0,998 | 109,0 | 6,21 |
| Citric acid | 3,56 | 0,999 | 98,3 | 1,81 |
| Ethylmalonic acid | 0,34 | 0,999 | 94,5 | 8,88 |
| Fumaric acid | 1,50 | 0,992 | 60,69 | 6,47 |
| Glutaconic acid | 2,88 | 0,999 | 101,7 | 13,00 |
| Glutaric acid | 2,47 | 0,999 | 103,9 | 3,56 |
| Glycolic acid | 3,25 | 0,998 | 122,61 | 5,51 |
| hexanoyl glycine | 0,06 | 0,999 | 96,77 | 4,17 |
| Homogentisic acid | 0,05 | 0,996 | 88,64 | 6,87 |
| Lactic acid | 1,38 | 0,997 | 83,2 | 6,73 |
| Malic acid | 4,97 | 0,995 | 74,36 | 2,14 |
| Malonic acid | 0,37 | 0,999 | 94,84 | 8,36 |
| Methylmalonic acid | 0,60 | 0,999 | 103,2 | 0,57 |
| N-(3-phenyl propionyl) glycine | 0,03 | 0,998 | 79,61 | 2,64 |
| N-Acetylaspartic acid | 0,26 | 0,999 | 75,66 | 6,22 |
| N-Acetyltyrosine | 0,08 | 0,999 | 96,83 | 5,95 |
| N-isovaleryl glycine | 0,18 | 0,999 | 85,58 | 7,27 |
| Orotic acid | 0,64 | 0,992 | 61,0 | 12,74 |
| Oxoproline | 2,55 | 0,997 | 85,47 | 4,32 |
| Phenyl pyrivuc acid | 0,14 | 0,999 | 104,5 | 7,43 |
| P-OH-phenyl lactic acid | 0,17 | 0,997 | 87,79 | 8,46 |
| Propionyl glycine | 0,20 | 0,993 | 71,02 | 9,06 |
| Pyruvic acid | 3,27 | 0,938 | 102,0 | 13,25 |
| Sebacic acid | 0,11 | 0,999 | 114,26 | 4,25 |
| Suberic acid | 0,13 | 0,999 | 114,56 | 4,77 |
| Suberylglycine | 0,18 | 0,999 | 93,12 | 1,82 |
| Succinic acid | 5,85 | 0,999 | 92,6 | 6,88 |
| Succunylacetone | 0,07 | 0,999 | 96,25 | 3,67 |
| Tiglyl glycine | 0,12 | 0,999 | 99,3 | 6,94 |