Organic Acid in Serum LC-MS/MS Analysis Kit
Human urine is a complex biofluid of diverse small polar metabolites such as organic acids. Organic acidurias are inborn errors of metabolism given rise to by defections of inherited enzymes which lead to accumulation excess amounts of normal organic acids in human urine. For this reason qualitative and quantitative analyses of urinary organic acids are prominent diagnostic tools for the interpretation of metabolite profile that reflects inherited metabolic disorders.
“Jasem-Organic Acids” method overcomes abovementioned drawbacks by using liquid chromatography tandem mass spectrometry (LC-MS/MS). Jasem method ensures quantification of 54 organic acids by absence of derivatization simplified sample preperation to diluting. Jasem offers two panels achieving sufficient chromatographic separation for crucial isomers by LC-MS/MS.
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Chromatogram
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METHOD PERFORMANCE
Analytes
LOQ
(ppm)
Ultivo
Linearity
(R2)
Ultivo
Recovery
Recovery %
Ultivo
Repeatability
% RSD
Ultivo
2-Hydroxy isovaleric acid
0,24
0,999
101,6
6,07
2-Metyl-citrate
0,55
0,997
118,92
2,17
2-OH-3-Methyl pentanoic acid
3,56
0,999
100,2
9,11
2-OH-Butyric acid
2,33
0,997
90,71
7,97
2-OH-glutaric acid
3,87
0,998
85,2
3,47
2-OH-isocaproic acid
0,50
0,999
100,8
12,29
2-OH-Phenyl acetic acid
0,07
0,999
94,05
4,92
2-Oxoadipic acid
0,18
0,999
106,25
13,01
3-Hydroxyisovaleric acid
0,67
0,999
94,5
7,96
3-Hydroxypropanoic acid
1,37
0,994
51,0
8,39
3-Methyl -2-oxovaleric acid
0,99
0,995
89,9
3,96
3-Methyl crotonyl glycine
0,33
0,999
103,5
7,76
3-Methylglutaconic acid
0,21
0,999
96,02
2,98
3-Methylglutaric acid
0,26
0,999
100,5
8,34
3-OH-2methyl butanoic acid
0,23
0,999
103,51
8,63
3-OH-3-Methyl-glutaric acid
0,79
0,999
98,78
4,08
3-OH-butyric acid
4,01
0,998
99,33
3,73
3-OH-glutaric acid
0,33
0,999
118,0
3,25
3-OH-Isobutyrate
2,25
0,998
83,32
4,35
3-OH-pentanoic acid
0,15
0,999
98,4
8,97
3-Phenyl lactic acid
0,09
0,998
91,14
3,92
4-Methyl-2-oxovaleric acid
0,64
0,999
94,1
10,89
4-OH-Phenyl-Acetic acid
3,00
0,999
92,06
11,87
4-OH-phenylpyruvic acid
3,74
0,994
74,0
1,54
Adipic acid
0,78
0,998
84,0
9,36
Alpha-ketoglutaric acid
3,60
0,998
109,0
6,21
Citric acid
3,56
0,999
98,3
1,81
Ethylmalonic acid
0,34
0,999
94,5
8,88
Fumaric acid
1,50
0,992
60,69
6,47
Glutaconic acid
2,88
0,999
101,7
13,00
Glutaric acid
2,47
0,999
103,9
3,56
Glycolic acid
3,25
0,998
122,61
5,51
hexanoyl glycine
0,06
0,999
96,77
4,17
Homogentisic acid
0,05
0,996
88,64
6,87
Lactic acid
1,38
0,997
83,2
6,73
Malic acid
4,97
0,995
74,36
2,14
Malonic acid
0,37
0,999
94,84
8,36
Methylmalonic acid
0,60
0,999
103,2
0,57
N-(3-phenyl propionyl) glycine
0,03
0,998
79,61
2,64
N-Acetylaspartic acid
0,26
0,999
75,66
6,22
N-Acetyltyrosine
0,08
0,999
96,83
5,95
N-isovaleryl glycine
0,18
0,999
85,58
7,27
Orotic acid
0,64
0,992
61,0
12,74
Oxoproline
2,55
0,997
85,47
4,32
Phenyl pyrivuc acid
0,14
0,999
104,5
7,43
P-OH-phenyl lactic acid
0,17
0,997
87,79
8,46
Propionyl glycine
0,20
0,993
71,02
9,06
Pyruvic acid
3,27
0,938
102,0
13,25
Sebacic acid
0,11
0,999
114,26
4,25
Suberic acid
0,13
0,999
114,56
4,77
Suberylglycine
0,18
0,999
93,12
1,82
Succinic acid
5,85
0,999
92,6
6,88
Succunylacetone
0,07
0,999
96,25
3,67
Tiglyl glycine
0,12
0,999
99,3
6,94
