Organic Acid in Urine LC-MS/MS Analysis Kit
Human urine is a complex biofluid of diverse small polar metabolites such as organic acids. Organic acidurias are inborn errors of metabolism given rise to by defections of inherited enzymes which lead to accumulation excess amounts of normal organic acids in human urine. For this reason qualitative and quantitative analyses of urinary organic acids are prominent diagnostic tools for the interpretation of metabolite profile that reflects inherited metabolic disorders.
“Jasem-Organic Acids” method overcomes abovementioned drawbacks by using liquid chromatography tandem mass spectrometry (LC-MS/MS). Jasem method ensures quantification of 54 organic acids by absence of derivatization simplified sample preperation to diluting. Jasem offers two panels achieving sufficient chromatographic separation for crucial isomers by LC-MS/MS.
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Chromatogram
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METHOD PERFORMANCE
NO
COMPOUNDS
LOQ(ppm) 6460 QQQ
LOQ (ppm) Ultivo
Linearity
Recovery (%)6460 QQQ
Repeatability
R2 6460 QQQ
RSD (%) 6460 QQQ
1
Alpha-Ketoglutaric acid
0,61
1,65
0,9979
118
1,74
2
2-Methyl citrate
0,72
1,22
0,9969
95,7
16,03
3
2-OH-3-Methylpentanoic acid
0,08
0,12
0,9986
104,2
6,98
4
2-OH-Butyric acid
0,06
0,28
0,9987
120
4,97
5
2-OH-Glutaric acid
0,37
0,84
0,9992
106,6
1,97
6
2-OH-Isocaproic acid
0,07
0,22
0,9992
108,4
5,66
7
2-OH-Isovaleric acid
0,07
0,06
0,9976
90,7
2,59
8
2-OH-Phenylacetic acid
0,07
0,06
0,9994
105,8
1,59
9
2-Oxoadipic acid
0,06
0,89
0,9983
111,8
7,78
10
3-Methyl -2-oxovaleric acid
0,13
1,88
0,9953
91,7
6,13
11
3-Methylcrotonylglycine
0,1
0,13
0,9998
96,9
2,99
12
3-Methylglutaconic acid
0,05
0,03
0,9987
104,2
2,8
13
3-Methylglutaric acid
0,08
0,04
0,999
87,1
3,38
14
3-OH-2-Methylbutanoic acid
0,09
0,51
0,9856
99,4
13,27
15
3-OH-3-Methylglutaric acid
0,18
0,99
0,9994
82,5
1,85
16
3-OH-Butyric acid
0,26
0,09
0,9991
113,5
8,84
17
3-OH-Glutaric acid
0,05
0,21
0,9996
115
2,7
18
3-OH-Isobutyrate
0,55
0,45
0,9994
94,4
1,74
19
3-OH-Isovaleric acid
0,11
0,16
0,9983
101,8
3,24
20
3-OH-Pentanoic acid
0,16
0,12
0,9996
94,14
6,95
21
3-OH-Propanoic acid
1,49
2,51
0,9983
88,4
4,55
22
3-Phenyllactic acid
0,06
0,07
0,999
115,9
6,34
23
4-Methyl-2-oxovaleric acid
0,15
1,92
0,9999
111,3
6,46
24
4-OH-Phenyl lactic acid
0,08
0,08
0,9968
77,3
10,34
25
4-OH-Phenylacetic acid
1,4
1,67
0,9997
110,1
2,01
26
4-OH-Phenylpyruvic acid
0,39
2,19
0,9977
115,8
6,03
27
Adipic acid
0,08
0,14
0,9993
100,2
6,01
28
Citric acid
0,39
1,91
0,9957
101,5
8,85
29
Ethylmalonic acid
0,05
0,2
0,9984
86,4
1,98
30
Fumaric acid
0,06
0,49
0,999
75,9
10,75
31
Glutaconic acid
0,2
0,5
0,9992
101,8
11,58
32
Glutaric acid
0,23
0,51
0,9999
93,6
2,45
33
Glycolic acid
0,72
5,51
0,9977
101,6
11,9
34
Hexanoylglycine
0,09
0,09
0,9985
116,5
3,08
35
Homogentisic acid
0,04
0,02
0,9993
78,7
3,19
36
Lactic acid
3,54
3,32
0,9997
106,4
10,8
37
Malic acid
0,06
0,18
0,9952
115
4,04
38
Malonic acid
0,07
0,36
0,9996
115
6,15
39
Methylmalonic acid
0,34
0,41
0,9998
87,6
3,27
40
N-(3-phenyl propionyl)glycine
0,05
0,07
0,9984
106,4
2,03
41
N-Acetylaspartic acid
0,1
0,1
0,9994
119
5,89
42
N-acetyltyrosine
0,13
0,06
0,9995
86,3
6,03
43
N-isovalerylglycine
0,07
0,11
0,9997
96
2,61
44
Orotic acid
0,04
0,82
0,9991
73,1
2,36
45
Oxoproline
0,05
0,07
0,9984
79,5
3,72
46
Phenylpyruvic acid
0,1
0,09
0,9995
98,3
6,3
47
Propionylglycine
0,18
0,15
0,9995
100,3
3,51
48
Pyruvic acid
0,22
4,98
0,9965
119
17,09
49
Sebacic acid
0,07
0,12
0,9998
94,5
2,4
50
Suberic acid
0,08
0,11
0,9993
94,9
1,99
51
Suberylglycine
0,13
0,09
0,9998
111,8
3,14
52
Succinic acid
0,1
0,11
0,9996
92,9
3,25
53
Succinylacetone
0,05
0,06
0,9999
102,9
4,24
54
Tiglylglycine
0,05
0,16
0,9999
93,5
4,74
