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Phenol, o-,p-Cresol in Urine HPLC Analysis Kit

Since the solvents that are frequently exposed in the industry are generally organic/petroleum-based, their use has been controlled because of deformations due to their toxic effects and exposure limits have been determined. It is mostly biotransformed after exposure and is excreted through urine. Metabolites obtained by bioconversion are often used as biomarkers for solvent exposure.

Benzene found in petroleum derivatives and cigarette smoke has been classified as a potential carcinogen type I by the International Agency for Research on Cancer (IARC). The toxicological effect of benzene is non-lymphocytic leukemia, aplastic anemia, chromosomal aberration and progressive degeneration of the bone marrow. t, t-muconic acid and phenol are known as urinary risk biomarkers of benzene exposure. Toluene exposure in cigarette smoke and automobile exhaust affects appetite and nervous system, therefore, causing fatigue and weakness, while high levels can cause unconsciousness and even death. o-Cresol and hippuric acid are known as the most important toluene exposure biomarkers.

  • Highlights

    Simple sample preparation without SPE or evaporation

    Total run time is 20.0 min

    Isocratic HPLC system with UV detector
  • Parameters

    Phenol, o-Cresol, p-Cresol
  • Matrix


Sample Preparation

  • Step 1
    The urine sample is diluted 3 times with distilled water
  • Step 2
    Pipet 500 μL of diluted sample into a screw cap tube
  • Step 3
    Add 50 μL of internal standard and 500 μL Reagent-1, vortex for 5 seconds
  • Step 4
    Incubated at 95 ̊C for 90 minutes
  • Step 5
    After cooling, add 500 μL Reagent-2 and centrifuge at 3500 rpm for 5 minutes.
  • Step 6
    Decant the clear supernatant into HPLC vial
  • Chromatogram

  • Method Performance

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