Phenol, o-,p-Cresol in Urine HPLC Analysis Kit
Since the solvents that are frequently exposed in the industry are generally organic/petroleum-based, their use has been controlled because of deformations due to their toxic effects and exposure limits have been determined. It is mostly biotransformed after exposure and is excreted through urine. Metabolites obtained by bioconversion are often used as biomarkers for solvent exposure.
Benzene found in petroleum derivatives and cigarette smoke has been classified as a potential carcinogen type I by the International Agency for Research on Cancer (IARC). The toxicological effect of benzene is non-lymphocytic leukemia, aplastic anemia, chromosomal aberration and progressive degeneration of the bone marrow. t, t-muconic acid and phenol are known as urinary risk biomarkers of benzene exposure. Toluene exposure in cigarette smoke and automobile exhaust affects appetite and nervous system, therefore, causing fatigue and weakness, while high levels can cause unconsciousness and even death. o-Cresol and hippuric acid are known as the most important toluene exposure biomarkers.
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Highlights
Simple sample preparation without SPE or evaporation
Total run time is 20.0 min
Isocratic HPLC system with UV detector -
Parameters
Phenol, o-Cresol, p-Cresol -
Matrix
Urine
Sample Preparation
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Step 1The urine sample is diluted 3 times with distilled water
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Step 2Pipet 500 μL of diluted sample into a screw cap tube
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Step 3Add 50 μL of internal standard and 500 μL Reagent-1, vortex for 5 seconds
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Step 4Incubated at 95 ̊C for 90 minutes
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Step 5After cooling, add 500 μL Reagent-2 and centrifuge at 3500 rpm for 5 minutes.
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Step 6Decant the clear supernatant into HPLC vial
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Chromatogram
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Method Performance