Skip to main content
PHENYLKETONURIA

PKU in Plasma/DBS HPLC Analysis Kit

Phenylketonuria, also called PKU, is a rare inherited disorder that causes an amino acid called phenylalanine to build up in the body. PKU is caused by a defect in the gene that helps create the enzyme needed to break down phenylalanine. Without the enzyme necessary to process phenylalanine, a dangerous buildup can develop when a person with PKU eats foods that contain protein or eats aspartame, an artificial sweetener. This can eventually lead to serious health problems. For the rest of their lives, people with PKU, need to follow a diet that limits phenylalanine, which is found mostly in foods that contain protein.

Babies are screened for PKU soon after birth. Recognizing PKU right away can help prevent major health problems.

  • Highlights

    Simple sample preparation without SPE or evaporation.

    11.0 minutes analysis time.

    Isocratic mobile phase gradient.
  • Parameters

    Phenylalanine, Tyrosine, Tryptophan
  • Matrix

    Plasma and Dried Blood Spot

Sample Preparation

For Plasma

  • Step 1
    Pipet 50 µL of plasma sample into a centrifuge tube
  • Step 2
    Add 300 μl of Reagent-1 and vortex for 15 seconds
  • Step 3
    Centrifuge at 10000-12000 rpm for 5 minutes
  • Step 4
    Decant the clear supernatant into HPLC vial
  • Step 5
  • Step 6
  • Step 7
  • Step 8
  • Step 9
  • Step 10
  • Step 11
  • Step 12

Sample Preparation

For DBS

  • Step 1
    Punch 3 mm of dried blood spot sample into a centrifuge tube
  • Step 2
    Add 200 μl of Reagent-1 and gently shake for 10 minutes
  • Step 3
    Decant the clear supernatant into HPLC vial.
  • Chromatogram

  • METHOD PERFORMANCE FOR PLASMA

    All results were obtained using Agilent UV dedector and 1200 series HPLC system in SEM R&D lab.

    Analytes

    LOQ

    (µg/L)

    Accuracy

    (%)

    Repeatability

    (%RSD)

    Linearity

    (R2)

    Phenylalanine

    0,3

    96,2

    3,2

    0,993

    Tyrosine

    0,3

    97,1

    3,8

    0,994

    Tryptophan

    0,6

    93,2

    4,9

    0,989

  • METHOD PERFORMANCE FOR DBS

    All results were obtained using Agilent UV dedector and 1200 series HPLC system in SEM R&D lab.

    Analytes

    LOQ

    (µg/L)

    Accuracy

    (%)

    Repeatability

    (%RSD)

    Linearity

    (R2)

    Phenylalanine

    0,6

    95,1

    3,9

    0,991

    Tyrosine

    0,6

    96,8

    4,1

    0,990

    Tryptophan

    0,9

    94,1

    5

    0,983

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..
PRODUCTS
Contact Us

The easiest way is to fill the contact form. Simply mail us to info@jasem.com.tr or give us a call +902165720200