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Phosphatidylethanol (PEth 16:0 18:1) in DBS LC-MS/MS Analysis Kit

Reliable biomarkers have great significance in the investigation of alcohol consumption patterns and in the treatment of alcohol-induced diseases. Conventional alcohol tests are based on the measurement of indirect biomarkers (GGT, ALT, AST, CDT etc.) to determine the toxic effect caused by alcohol consumption. However, these indirect biomarkers are not specific enough since they are also affected by physiological and pathological conditions. Direct biomarkers to detect alcohol intake are non-oxidative products of ethanol metabolism. Ethylglucuronide (EtG) and ethylsulfate (EtS) are conjugated metabolites of ethanol and have narrow detection windows in biofluids. Additionally, severe clinical errors in results may occur stemmed from post-sampling process, either due to storage circumstances or medical conditions of the person being sampled. Phosphatidylethanol (PEth), blood-based direct alcohol biomarker, represents a group of unnatural phospholipids formed on leukocytes and predominantly erythrocyte membranes. Studies demonstrate that the most dominant of these possible forms is PEth-16:0 18:1 consisting of a palmitic and an oleic acid. The presence of PEth in blood samples is an indisputable indicator of ethanol consumption

  • Highlights

    Just a few pipetting steps for sample preparation; “extract and shoot.”

    Quantitative analysis of PEth 16:0 18:1 in 5 min. for DBS specimens

    Safeguarded by its own stable labelled isotope as internal standard

    Consuming small volume of patient’s sample. Suitable for microsampling devices

    Long life span of HPLC column
  • Parameters

    Phosphatidylethanol (PEth 16:0 18:1)
  • Matrix

    Dried Blood Spot (DBS)

Sample Preparation

  • Step 1
    Punch 6 mm of blood spot into a conical microtube
  • Step 2
    Add 100 μL of extraction reagent (containing internal standard-PEth 16:0 18:1 D5) and shake for 10 min. at room temperature
  • Step 3
    Pipette extract into a HPLC vial prior to injection
  • Chromatogram

  • Method Performance

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