
Serotonin in Serum/Plasma
Serotonin in Serum/Plasma LC-MS/MS Analysis Kit
Serotonin (5-hydroxytryptamine) is a monoamine neurotransmitter synthesized from the amino acid tryptophan. Most of total body serotonin is found in the gastrointestinal (GI) tract, where it modulates the functions of the bowel by controlling smooth muscle contraction. It also acts as a cardiovascular vasoconstriction mediator and in the brain as a neurotransmitter. Neuroendocrine neoplasms (NENs), which originate from GI tract enterochromaffin cells, may produce large amounts of serotonin.
Highlights
Total run time is 6 min.
Just a few pipetting steps for the sample treatment-protein precipitate and shoot approachno need for derivatization
Consuming small volume of patient’s sample
Long life span of HPLC column
Just a few pipetting steps for the sample treatment-protein precipitate and shoot approachno need for derivatization
Consuming small volume of patient’s sample
Long life span of HPLC column
Parameters
Serotonin
Matrix
Serum and Plasma
Sample Preparation
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Step 1Pipette 150 µL of serum sample/working calibration standard into a glass centrifuge tube.
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Step 2Add 20 µL of internal standard working stock solution, vortex for 5 sec.
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Step 3Add 250 µL of Reagent-1, additionally vortex for 15 sec. then centrifuge at 4000 rpm for 5 min.
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Step 4Decant the clear supernatant into HPLC vial prior to injection.
