Steroid Hormones in Serum LC-MS/MS Analysis Kit
Steroid hormones are defined as effective biological messengers which take part in crucial body processes even at low concentrations. Thus, reliable, and simultaneous analysis of a broad panel of steroid hormones plays vital role for the investigation of the hormone profile, which could be useful in detection endocrine disorders resulting from defects in steroid biosynthesis such as congenital adrenal hyperplasia (CAH). Due to the restrictions and shortcomings of immunoassay techniques for measurement of steroid hormones in body fluids, liquid chromatography tandem mass spectrometry (LC-MS/MS) is increasingly becoming the method for multi-class steroid hormone detection.
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Chromatogram
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METHOD PERFORMANCE
Analyte
LOQ (µg/L) 6460 QQQ
LOQ (µg/L) Ultivo QQQ
Accuracy % 6460 QQQ
Repeatabilty % CV 6460 QQQ
Linearity R2 6460 QQQ
11-Deoxycorticosterone
0,016
0,123
95.34
6.84
0.994
11-Deoxycortisol
0,015
0,049
102.23
4.45
0.998
17-Hydroxypregnenolone
0,145
1,251
100.44
9.24
0.997
17-Hydroxyprogesterone
0,028
0,078
97.01
4.80
0.997
4-Androstene 3,17-dione
0,023
0,193
109.93
1.99
0.998
Aldosterone
0,011
0,100
99.81
6.34
0.998
Androsterone
0,035
0,235
93.70
13
0.994
Corticosterone
0,054
0,424
98.42
4.09
0.998
Cortisol
0,102
0,694
89.74
1.69
0.999
DHEA
0,102
0,328
109.62
6.66
0.998
DHEAS
4,945
38,012
103.47
2.63
0.997
Dihydrotestosterone
0,012
0,263
101.98
14.36
0.997
Estradiol (Oestradiol)
0,015
0,227
109.37
6.32
0.998
Pregnenolone
0,033
0,370
77.49
10.46
0.994
Progesterone
0,033
0,079
108.37
4.45
0.994
Testosterone
0,008
0,027
96.75
1.80
0.997
