Steroid Hormones in Serum LC-MS/MS Analysis Kit

Steroid hormones are defined as effective biological messengers which take part in crucial body processes even at low concentrations. Thus, reliable, and simultaneous analysis of a broad panel of steroid hormones plays vital role for the investigation of the hormone profile, which could be useful in detection endocrine disorders resulting from defects in steroid biosynthesis such as congenital adrenal hyperplasia (CAH). Due to the restrictions and shortcomings of immunoassay techniques for measurement of steroid hormones in body fluids, liquid chromatography tandem mass spectrometry (LC-MS/MS) is increasingly becoming the method for multi-class steroid hormone detection.

  • Highlights

    Analysis of clinically relevant 16 steroid hormones in a single injection-no need for separate injections.

    Total run time is 16.0 min.

    One sample preparation for all steroid hormones. Easy liquid-liquid extraction step following by protein precipitation for the sample treatment-no need for SPE.

    Covered corresponding isotope labeled internal standards of important steroid hormones-11 ISs.

    Long life span of HPLC column.
  • Parameters

    11-Deoxycorticosterone, 11-Deoxycortisol, 17-α-Hydroxypregnenolone, 17-α-Hydroxyprogesterone, Aldosterone, Androstene-3,17-dione, Androsterone, Corticosterone, Cortisol, DHEA, DHEAS, Dihydrotestosterone, Estradiol, Pregnenolone, Progesterone and Testosterone.
  • Matrix


Sample Preparation

Register to view this area

This content has been restricted to logged in users only. Please login to view this content.

Register to view chromatogram and method performance

This content has been restricted to logged in users only. Please login to view this content.
Contact Us

The easiest way is to fill the contact form. Simply mail us to or give us a call +902165720200