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Sulfonamides in Milk

Sulfonamides in Milk Lc-Ms/Ms Analysis Kit

Sulfonamides belong to a large and important class of antibacterial drugs and are very common in veterinary and human medicine.

Sulfonamides are illegally used as additives in animal feed because they may have a growth-promoting effect.

The milk and dairy products industries are among the sectors most affected by the presence of antibiotic residues, not only because of the risk of chemical poisoning, allergic reactions, and the development of mechanisms of bacterial resistance, causing a serious threat to human and animal health, but also because of important economic losses derived by the inhibitory effect of these biocides in the fermentation processes (production of cheese and cultured milk products).

Highlights

Quick and simple sample preparation steps without SPE or evaporation.

Shorter run time.

Parameters

at the bottom of the page

Matrix

Milk

Sample Preparation


  • Step 1
    Accurately transfer by pipetting 1 ml of milk sample into a glass experiment tube.
  • Step 2
    Add 1 ml of Reagent 1 and vortex it 30 seconds, Centrifuge at 2000 rpm for 3 minutes.
  • Step 3
    Transfer 1 ml from supernatant to a clean centrifuge tube.
  • Step 4
    Add 4 ml Reagent 2 onto sample and vortex 30 seconds.
  • Step 5
    Filter out from 0.45 µm RC filters,Directly inject into the system.

Chromatogram

METHOD PERFORMANCE

Analytes

LOQ*(ppb) 6460 QQQ

Accuracy (%) 6460 QQQ

Repeatability (%RSD) 6460 QQQ

Linearity (R2) 6460 QQQ

Sulfadiazin

8.86

102.60

3.74

0.999

Sulfathiazole

7.15

102.46

2.65

0.998

Sulfapyridine

5.62

101.67

4.19

0.998

Sulfamerazine

7.88

101.52

2.90

0.998

Sulfamethazine

6.38

95.37

2.15

0.999

Sulfachloropyridazine

8.67

102.57

5.36

0.999

Sulfadoxin

5.55

90.69

2.75

0.998

Sulfamethoxazole

5.74

90.86

7.44

0.997

Sulfaquinoxaline

8.29

80.37

6.12

0.996

Sulfadimethoxine

5.23

97.68

2.60

0.999

* LOQ: Limit of quantification, 10sd + AVERAGE

Sulfadiazin,Sulfathiazole,Sulfapyridine,Sulfamerazine,Sulfamethazine,Sulfachloropyridazine, Sulfadoxin, Sulfamethoxazole, Sulfaquinoxaline, Sulfadimethoxine
ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..
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