
Sulfonamides in Milk Lc-Ms/Ms Analysis Kit
Sulfonamides belong to a large and important class of antibacterial drugs and are very common in veterinary and human medicine.
Sulfonamides are illegally used as additives in animal feed because they may have a growth-promoting effect.
The milk and dairy products industries are among the sectors most affected by the presence of antibiotic residues, not only because of the risk of chemical poisoning, allergic reactions, and the development of mechanisms of bacterial resistance, causing a serious threat to human and animal health, but also because of important economic losses derived by the inhibitory effect of these biocides in the fermentation processes (production of cheese and cultured milk products).
Highlights
Shorter run time.
Parameters
Matrix
Sample Preparation
-
Step 1Accurately transfer by pipetting 1 ml of milk sample into a glass experiment tube.
-
Step 2Add 1 ml of Reagent 1 and vortex it 30 seconds, Centrifuge at 2000 rpm for 3 minutes.
-
Step 3Transfer 1 ml from supernatant to a clean centrifuge tube.
-
Step 4Add 4 ml Reagent 2 onto sample and vortex 30 seconds.
-
Step 5Filter out from 0.45 µm RC filters,Directly inject into the system.

Chromatogram
METHOD PERFORMANCE
Analytes | LOQ*(ppb) 6460 QQQ | Accuracy (%) 6460 QQQ | Repeatability (%RSD) 6460 QQQ | Linearity (R2) 6460 QQQ |
Sulfadiazin | 8.86 | 102.60 | 3.74 | 0.999 |
Sulfathiazole | 7.15 | 102.46 | 2.65 | 0.998 |
Sulfapyridine | 5.62 | 101.67 | 4.19 | 0.998 |
Sulfamerazine | 7.88 | 101.52 | 2.90 | 0.998 |
Sulfamethazine | 6.38 | 95.37 | 2.15 | 0.999 |
Sulfachloropyridazine | 8.67 | 102.57 | 5.36 | 0.999 |
Sulfadoxin | 5.55 | 90.69 | 2.75 | 0.998 |
Sulfamethoxazole | 5.74 | 90.86 | 7.44 | 0.997 |
Sulfaquinoxaline | 8.29 | 80.37 | 6.12 | 0.996 |
Sulfadimethoxine | 5.23 | 97.68 | 2.60 | 0.999 |
* LOQ: Limit of quantification, 10sd + AVERAGE