Sulfonamides in Milk Lc-Ms/Ms Analysis Kit
Sulfonamides belong to a large and important class of antibacterial drugs and are very common in veterinary and human medicine.
Sulfonamides are illegally used as additives in animal feed because they may have a growth-promoting effect.
The milk and dairy products industries are among the sectors most affected by the presence of antibiotic residues, not only because of the risk of chemical poisoning, allergic reactions, and the development of mechanisms of bacterial resistance, causing a serious threat to human and animal health, but also because of important economic losses derived by the inhibitory effect of these biocides in the fermentation processes (production of cheese and cultured milk products).
-
Highlights
Quick and simple sample preparation steps without SPE or evaporation.
Shorter run time. -
Parameters
at the bottom of the page -
Matrix
Milk
Sample Preparation
-
Step 1Accurately transfer by pipetting 1 ml of milk sample into a glass experiment tube.
-
Step 2Add 1 ml of Reagent 1 and vortex it 30 seconds, Centrifuge at 2000 rpm for 3 minutes.
-
Step 3Transfer 1 ml from supernatant to a clean centrifuge tube.
-
Step 4Add 4 ml Reagent 2 onto sample and vortex 30 seconds.
-
Step 5Filter out from 0.45 µm RC filters,Directly inject into the system.
-
Chromatogram
-
METHOD PERFORMANCE
Analytes
LOQ*(ppb) 6460 QQQ
Accuracy (%) 6460 QQQ
Repeatability (%RSD) 6460 QQQ
Linearity (R2) 6460 QQQ
Sulfadiazin
8.86
102.60
3.74
0.999
Sulfathiazole
7.15
102.46
2.65
0.998
Sulfapyridine
5.62
101.67
4.19
0.998
Sulfamerazine
7.88
101.52
2.90
0.998
Sulfamethazine
6.38
95.37
2.15
0.999
Sulfachloropyridazine
8.67
102.57
5.36
0.999
Sulfadoxin
5.55
90.69
2.75
0.998
Sulfamethoxazole
5.74
90.86
7.44
0.997
Sulfaquinoxaline
8.29
80.37
6.12
0.996
Sulfadimethoxine
5.23
97.68
2.60
0.999
* LOQ: Limit of quantification, 10sd + AVERAGE
-
Sulfadiazin,Sulfathiazole,Sulfapyridine,Sulfamerazine,Sulfamethazine,Sulfachloropyridazine, Sulfadoxin, Sulfamethoxazole, Sulfaquinoxaline, Sulfadimethoxine