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Sulfonamides in Milk

Sulfonamides in Milk Lc-Ms/Ms Analysis Kit

Sulfonamides belong to a large and important class of antibacterial drugs and are very common in veterinary and human medicine.

Sulfonamides are illegally used as additives in animal feed because they may have a growth-promoting effect.

The milk and dairy products industries are among the sectors most affected by the presence of antibiotic residues, not only because of the risk of chemical poisoning, allergic reactions, and the development of mechanisms of bacterial resistance, causing a serious threat to human and animal health, but also because of important economic losses derived by the inhibitory effect of these biocides in the fermentation processes (production of cheese and cultured milk products).

  • Highlights

    Quick and simple sample preparation steps without SPE or evaporation.

    Shorter run time.
  • Parameters

    at the bottom of the page
  • Matrix

    Milk

Sample Preparation


  • Step 1
    Accurately transfer by pipetting 1 ml of milk sample into a glass experiment tube.
  • Step 2
    Add 1 ml of Reagent 1 and vortex it 30 seconds, Centrifuge at 2000 rpm for 3 minutes.
  • Step 3
    Transfer 1 ml from supernatant to a clean centrifuge tube.
  • Step 4
    Add 4 ml Reagent 2 onto sample and vortex 30 seconds.
  • Step 5
    Filter out from 0.45 µm RC filters,Directly inject into the system.
  • Chromatogram

  • METHOD PERFORMANCE

    Analytes

    LOQ*(ppb) 6460 QQQ

    Accuracy (%) 6460 QQQ

    Repeatability (%RSD) 6460 QQQ

    Linearity (R2) 6460 QQQ

    Sulfadiazin

    8.86

    102.60

    3.74

    0.999

    Sulfathiazole

    7.15

    102.46

    2.65

    0.998

    Sulfapyridine

    5.62

    101.67

    4.19

    0.998

    Sulfamerazine

    7.88

    101.52

    2.90

    0.998

    Sulfamethazine

    6.38

    95.37

    2.15

    0.999

    Sulfachloropyridazine

    8.67

    102.57

    5.36

    0.999

    Sulfadoxin

    5.55

    90.69

    2.75

    0.998

    Sulfamethoxazole

    5.74

    90.86

    7.44

    0.997

    Sulfaquinoxaline

    8.29

    80.37

    6.12

    0.996

    Sulfadimethoxine

    5.23

    97.68

    2.60

    0.999

    * LOQ: Limit of quantification, 10sd + AVERAGE

  • Sulfadiazin,Sulfathiazole,Sulfapyridine,Sulfamerazine,Sulfamethazine,Sulfachloropyridazine, Sulfadoxin, Sulfamethoxazole, Sulfaquinoxaline, Sulfadimethoxine
ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..
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