
Sulfonamides In Tissue LC-MS/MS Analysis Kit
Sulfonamides are one of the oldest groups of pharmacologically active substances used in veterinary medicine.Sulfonamides are illegally used as additives in animal feed because they may have a growth-promoting effect.
Sulfonamides generate potentially serious problems in human health, such as allergic or toxic reactions.Furthermore, the main risk from the excessive use of antimicrobials in animals is that bacteria may develop resistance.
In addition, some sulfonamides have been found to be potentially carcinogenic and this fact has become a cause for considerable debate in food safety.
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Highlights
Easy sample preparation without the need for solid phase extraction (SPE) and additional concentration steps -
Parameters
at the bottom of the page -
Matrix
Poultry and Red meat tissues
Sample Preparation
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Step 1Weigh 5 g of minced/grinded meat sample into a 50 ml polypropylene centrifuge tube.
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Step 2Add 62.5 µl of 10 ppm ISTD onto meat sample.
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Step 3Add 10 ml of Reagent 1 and immediately cap and vortex 10 minutes vigorously.
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Step 4Centrifuge at 3000 rpm for 5 minutes.
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Step 5Pipette 1 ml from supernatant into a clean glass centrifuge tube.
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Step 6Add 1.5 ml Reagent 2 and dissolve the residue and vortex 1 minute.
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Step 7Filter out from 0.45 µm RC filters into HPLC vial.
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Chromatogram
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METHOD PERFORMANCE
Analytes
LOQ (ppb) 6460 QQQ
Accuracy (%) 6460 QQQ
Repeatability (%RSD) 6460 QQQ
Linearity (R2) 6460 QQQ
Sulfadiazin
7. 81
80.14
3.67
0.999
Sulfathiazole
5.33
80.12
3.54
0.999
Sulfapyridine
7.19
79.08
6.85
0.999
Sulfamerazine
6.31
87.24
5.01
0.999
Sulfamethazine
5.11
92.54
2.58
0.999
Sulfachloropyridazine
7.45
96.07
2.99
0.999
Sulfadoxin
6.59
92.68
2.70
0.999
Sulfamethoxazole
6.4
95.10
2.94
0.998
Sulfaquinoxaline
7.33
90.96
3.84
0.998
Sulfadimethoxine
5.26
94.79
2.03
0.998
* LOQ: Limit of quantification, 10sd + AVERAGE
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Sulfadiazin,Sulfathiazole,Sulfapyridine,Sulfamerazine,Sulfamethazine,Sulfachloropyridazine,Sulfadoxin, Sulfamethoxazole, Sulfaquinoxaline, Sulfadimethoxine