Sulfonamides In Tissue

Sulfonamides In Tissue LC-MS/MS Analysis Kit

Sulfonamides are one of the oldest groups of pharmacologically active substances used in veterinary medicine.Sulfonamides are illegally used as additives in animal feed because they may have a growth-promoting effect.

Sulfonamides generate potentially serious problems in human health, such as allergic or toxic reactions.Furthermore, the main risk from the excessive use of antimicrobials in animals is that bacteria may develop resistance.

In addition, some sulfonamides have been found to be potentially carcinogenic and this fact has become a cause for considerable debate in food safety.

Highlights

Easy sample preparation without the need for solid phase extraction (SPE) and additional concentration steps
Parameters

at the bottom of the page
Matrix

Poultry and Red meat tissues

Sample Preparation

Step 1

Weigh 5 g of minced/grinded meat sample into a 50 ml polypropylene centrifuge tube.
Step 2

Add 62.5 µl of 10 ppm ISTD onto meat sample.
Step 3

Add 10 ml of Reagent 1 and immediately cap and vortex 10 minutes vigorously.
Step 4

Centrifuge at 3000 rpm for 5 minutes.
Step 5

Pipette 1 ml from supernatant into a clean glass centrifuge tube.
Step 6

Add 1.5 ml Reagent 2 and dissolve the residue and vortex 1 minute.
Step 7

Filter out from 0.45 µm RC filters into HPLC vial.

Chromatogram

METHOD PERFORMANCE

Analytes	LOQ (ppb) 6460 QQQ	Accuracy (%) 6460 QQQ	Repeatability (%RSD) 6460 QQQ	Linearity (R²) 6460 QQQ
Sulfadiazin	7. 81	80.14	3.67	0.999
Sulfathiazole	5.33	80.12	3.54	0.999
Sulfapyridine	7.19	79.08	6.85	0.999
Sulfamerazine	6.31	87.24	5.01	0.999
Sulfamethazine	5.11	92.54	2.58	0.999
Sulfachloropyridazine	7.45	96.07	2.99	0.999
Sulfadoxin	6.59	92.68	2.70	0.999
Sulfamethoxazole	6.4	95.10	2.94	0.998
Sulfaquinoxaline	7.33	90.96	3.84	0.998
Sulfadimethoxine	5.26	94.79	2.03	0.998

* LOQ: Limit of quantification, 10sd + AVERAGE

Parameters

Sulfadiazin,Sulfathiazole,Sulfapyridine,Sulfamerazine,Sulfamethazine,Sulfachloropyridazine,Sulfadoxin, Sulfamethoxazole, Sulfaquinoxaline, Sulfadimethoxine