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Sulfonamides In Tissue

Sulfonamides In Tissue LC-MS/MS Analysis Kit

Sulfonamides are one of the oldest groups of pharmacologically active substances used in veterinary medicine.Sulfonamides are illegally used as additives in animal feed because they may have a growth-promoting effect.

Sulfonamides generate potentially serious problems in human health, such as allergic or toxic reactions.Furthermore, the main risk from the excessive use of antimicrobials in animals is that bacteria may develop resistance.

In addition, some sulfonamides have been found to be potentially carcinogenic and this fact has become a cause for considerable debate in food safety.

  • Highlights

    Easy sample preparation without the need for solid phase extraction (SPE) and additional concentration steps
  • Parameters

    at the bottom of the page
  • Matrix

    Poultry and Red meat tissues

Sample Preparation


  • Step 1
    Weigh 5 g of minced/grinded meat sample into a 50 ml polypropylene centrifuge tube.
  • Step 2
    Add 62.5 µl of 10 ppm ISTD onto meat sample.
  • Step 3
    Add 10 ml of Reagent 1 and immediately cap and vortex 10 minutes vigorously.
  • Step 4
    Centrifuge at 3000 rpm for 5 minutes.
  • Step 5
    Pipette 1 ml from supernatant into a clean glass centrifuge tube.
  • Step 6
    Add 1.5 ml Reagent 2 and dissolve the residue and vortex 1 minute.
  • Step 7
    Filter out from 0.45 µm RC filters into HPLC vial.
  • Chromatogram

  • METHOD PERFORMANCE

    Analytes

    LOQ (ppb) 6460 QQQ

    Accuracy (%) 6460 QQQ

    Repeatability (%RSD) 6460 QQQ

    Linearity (R2) 6460  QQQ

    Sulfadiazin

    7. 81

    80.14

    3.67

    0.999

    Sulfathiazole

    5.33

    80.12

    3.54

    0.999

    Sulfapyridine

    7.19

    79.08

    6.85

    0.999

    Sulfamerazine

    6.31

    87.24

    5.01

    0.999

    Sulfamethazine

    5.11

    92.54

    2.58

    0.999

    Sulfachloropyridazine

    7.45

    96.07

    2.99

    0.999

    Sulfadoxin

    6.59

    92.68

    2.70

    0.999

    Sulfamethoxazole

    6.4

    95.10

    2.94

    0.998

    Sulfaquinoxaline

    7.33

    90.96

    3.84

    0.998

    Sulfadimethoxine

    5.26

    94.79

    2.03

    0.998

    * LOQ: Limit of quantification, 10sd + AVERAGE

  • Sulfadiazin,Sulfathiazole,Sulfapyridine,Sulfamerazine,Sulfamethazine,Sulfachloropyridazine,Sulfadoxin, Sulfamethoxazole, Sulfaquinoxaline, Sulfadimethoxine
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