
Tetracyclines In Honey LC-MS/MS Analysis Kit
Tetracyclines (TCs) are broad-spectrum antibiotics, widely used in animal husbandry and apiculture for prevention and treatment of diseases and as growth promotion.
Antibiotics are found in honey largely because they are used in apiculture for treatment of bacterial diseases. Oxytetracycline is commonly used to treat European foulbrood disease (EFB) and American foulbrood diseases (AFB) caused by Paenibacilus (Bacillus) larvae and Streptococcus pluton bacteria, respectively.
Highlights
Shorter run time.
Parameters
Matrix
Sample Preparation
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Step 1Accurately weigh 1 g of honey sample into a glass experiment tube
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Step 2Add 100 µl of 1 ppm ISTD onto honey sample and vortex 10 seconds.
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Step 3Add 3 ml of Reagent 1 and vortex 30 seconds vigorously.
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Step 4Filter out from 0.45 µm RC filters,Directly inject into the system.

Chromatogram
METHOD PERFORMANCE
Analytes | LOQ*(ppb) 6460 QQQ | Accuracy (%) 6460 QQQ | Repeatability (%RSD) 6460 QQQ | Linearity (R2) 6460 QQQ |
Chlortetracycline | 3.50 | 104.51 | 3.94 | 0.9998 |
Oxytetracycline | 4.24 | 107.59 | 2.64 | 0.9994 |
Tetracycline | 4.27 | 117.07 | 3.25 | 0.9998 |
Doxycycline | 4.75 | 104.35 | 2.42 | 0.9994 |
* LOQ: Limit of quantification, 10sd + AVERAGE