Tetracyclines In Honey

Tetracyclines In Honey LC-MS/MS Analysis Kit

Tetracyclines (TCs) are broad-spectrum antibiotics, widely used in animal husbandry and apiculture for prevention and treatment of diseases and as growth promotion.

Antibiotics are found in honey largely because they are used in apiculture for treatment of bacterial diseases. Oxytetracycline is commonly used to treat European foulbrood disease (EFB) and American foulbrood diseases (AFB) caused by Paenibacilus (Bacillus) larvae and Streptococcus pluton bacteria, respectively.

Highlights

Easy sample preparation without the need for solid phase extraction (SPE) and additional concentration steps

Shorter run time.
Parameters

Chlortetracycline, Oxytetracycline, Tetracycline, Doxycycline
Matrix

Honey

Sample Preparation

Step 1

Accurately weigh 1 g of honey sample into a glass experiment tube
Step 2

Add 100 µl of 1 ppm ISTD onto honey sample and vortex 10 seconds.
Step 3

Add 3 ml of Reagent 1 and vortex 30 seconds vigorously.
Step 4

Filter out from 0.45 µm RC filters,Directly inject into the system.

Chromatogram

METHOD PERFORMANCE

Analytes	LOQ*(ppb) 6460 QQQ	Accuracy (%) 6460 QQQ	Repeatability (%RSD) 6460 QQQ	Linearity (R²) 6460 QQQ
Chlortetracycline	3.50	104.51	3.94	0.9998
Oxytetracycline	4.24	107.59	2.64	0.9994
Tetracycline	4.27	117.07	3.25	0.9998
Doxycycline	4.75	104.35	2.42	0.9994

* LOQ: Limit of quantification, 10sd + AVERAGE