BUSULFAN & METHOTREXATE KIT

BUSULFAN & METHOTREXATE IN SERUM, PLASMA

The efficacy and toxicity of anticancer drugs are related to parent drug and / or metabolite concentrations in body fluids and tissues. Therefore monitoring of anticancer drugs in biological fluids and tissues is important during both pre-clinical and clinical development and often in routine clinical use.

Jasem method provides analysis of busulfan and methotrexate in serum by LC-MS/MS only in 5 min.

  • Features

    • Quick and simple sample preparation steps
    • No need SPE cleaning for samples
    • Allows to analyse both drugs in same injection
    • Shorter sample prep and instrument run time
  • parameters

    • Busulfan
    • Methotrexate
  • Matrix

    • Serum
    • Plasma

Sample Preparation


  • Step 1

    Pipet 100 µl of serum sample into a clean centrifuge tube and add 700 µl of precipitation reagent into sample1

  • Step 2

    Vortex for 15 seconds and centrifuge at 3000 rpm for 5 min

  • Step 3

    Decant the suprnatant into an HPLC vial

  • Example chromatogram

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..

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Contact Us

The easiest way is to fill the contact form. Simply mail us to info@jasem.com.tr or give us a call +902165720200