The efficacy and toxicity of anticancer drugs are related to parent drug and / or metabolite concentrations in body fluids and tissues. Therefore monitoring of anticancer drugs in biological fluids and tissues is important during both pre-clinical and clinical development and often in routine clinical use.
Jasem method provides analysis of busulfan and methotrexate in serum by LC-MS/MS only in 5 min.
Pipet 100 µl of serum sample into a clean centrifuge tube and add 700 µl of precipitation reagent into sample1
Vortex for 15 seconds and centrifuge at 3000 rpm for 5 min
Decant the suprnatant into an HPLC vial