BUSULFAN & METHOTREXATE KIT

BUSULFAN & METHOTREXATE IN SERUM/PLASMA
LC-MS/MS KIT

The efficacy and toxicity of anticancer drugs are related to parent drug and/or metabolite concentrations in body fluids and tissues. Therefore, monitoring of anticancer drugs in biological fluids and tissues is important during both pre-clinical and clinical development and often in routine clinical use.

  • Highlights

    • Simultaneously quantification of Busulfan and Methotrexate
    • Total run time 5 min.
    • Just a few pipetting steps for the sample treatment-protein precipitate and shoot approachno need for derivatization
    • Consuming small volume of patient’s sample
    • Long life span of HPLC column
  • parameters

    • Busulfan
    • Methotrexate
  • Matrix

    • Serum
    • Plasma

Sample Preparation


  • Step 1

    Pipette 100 µl serum sample/calibrators in a centrifuge tube

  • Step 2

    Add 50 µL of IS solution, vortex for 5 sec.

  • Step 3

    Add 650 µl Reagent-1 to the sample and vortex 30 sec.

  • Step 4

    Centrifuge 3000 rpm for 5 min. and decant the supernatant into a HPLC via

  • Example chromatogram

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..

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