BUSULFAN & METHOTREXATE

BUSULFAN & METHOTREXATE KIT

BUSULFAN & METHOTREXATE IN SERUM/PLASMA
LC-MS/MS KIT

The efficacy and toxicity of anticancer drugs are related to parent drug and/or metabolite concentrations in body fluids and tissues. Therefore, monitoring of anticancer drugs in biological fluids and tissues is important during both pre-clinical and clinical development and often in routine clinical use.

Highlights
- Simultaneously quantification of Busulfan and Methotrexate
- Total run time 5 min.
- Just a few pipetting steps for the sample treatment-protein precipitate and shoot approachno need for derivatization
- Consuming small volume of patient’s sample
- Long life span of HPLC column
parameters
- Busulfan
- Methotrexate
Matrix
- Serum
- Plasma

Sample Preparation

Step 1

Pipette 100 µl serum sample/calibrators in a centrifuge tube
Step 2

Add 50 µL of IS solution, vortex for 5 sec.
Step 3

Add 650 µl Reagent-1 to the sample and vortex 30 sec.
Step 4

Centrifuge 3000 rpm for 5 min. and decant the supernatant into a HPLC via