BUSULFAN & METHOTREXATE

BUSULFAN & METHOTREXATE KIT

BUSULFAN & METHOTREXATE IN SERUM, PLASMA

The efficacy and toxicity of anticancer drugs are related to parent drug and / or metabolite concentrations in body fluids and tissues. Therefore monitoring of anticancer drugs in biological fluids and tissues is important during both pre-clinical and clinical development and often in routine clinical use.

Jasem method provides analysis of busulfan and methotrexate in serum by LC-MS/MS only in 5 min.

Features
- Quick and simple sample preparation steps
- No need SPE cleaning for samples
- Allows to analyse both drugs in same injection
- Shorter sample prep and instrument run time
parameters
- Busulfan
- Methotrexate
Matrix
- Serum
- Plasma

Sample Preparation

Step 1

Pipet 100 µl of serum sample into a clean centrifuge tube and add 700 µl of precipitation reagent into sample1
Step 2

Vortex for 15 seconds and centrifuge at 3000 rpm for 5 min
Step 3

Decant the suprnatant into an HPLC vial