25-OH VITAMIN D2/D3 & EPI-VIT D3

25-OH VITAMIN D2/D3 & EPI-VIT D3 LC-MS/MS ANALYSIS KIT

Vitamin D plays a vital role in skeletal metabolism, calcium homeostasis, and also in the functioning of the immune, cardiovascular, and reproductive systems.

Vitamin D deficiency leads to rickets and osteomalacia and is also associated with breast and colorectal cancers, multiple sclerosis, dementia, rheumatoid arthritis, diabetes, Parkinson’s and Alzheimer’s diseases. Consequently, laboratory testing of serum vitamin D levels has experienced phenomenal growth over the past several years.

LC-MS/MS is currently the best technique available for the correct quantification of 25-Hydroxy Vitamin D3 and 25-Hydroxy Vitamin D2

  • Parameters

    • 25-OH Vitamin D2 and D3 & Epi-Vit D3  

  • Matrix

    • Serum & Plasma

Numune Hazırlık


  • Step 1

    Pipet 200 μL of serum sample into a glass centrifuge tube

  • Step 2

    Add 100 μL of Reagent-1, vortex for 5 seconds.

  • Step 3

    Add 25 μL of internal standard solution and additionally vortex for 5 seconds.

  • Step 4

    Add 975 μL of Reagent-2 and vortex for 15 seconds. Then, centrifuge at 3000 rpm for 5 minutes

  • Step 5

    Decant the supernatant into a HPLC vial prior to LC-MS/MS analysis.

  • Example Chromatogram

  • Method Performance

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..

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