25-Hydroxy Vıtamın D2/D3

VITAMIN D IN SERUM/PLASMA
LC-MS/MS KIT

Vitamin D plays a vital role in skeletal metabolism, calcium homeostasis, and also in the functioning of the immune, cardiovascular, and reproductive systems. Vitamin D deficiency leads to rickets and osteomalacia and is also associated with breast and colorectal cancers, multiple sclerosis, dementia, rheumatoid arthritis, diabetes, Parkinson’s and Alzheimer’s diseases. Consequently, laboratory testing of serum vitamin D levels has experienced phenomenal growth over the past several years. LC-MS/ MS is currently the best technique available for the correct quantification of 25-Hydroxy Vitamin D3 and 25-Hydroxy Vitamin D2

  • Highlights

    • Simultaneous measurement the concentrations of 25-Hydroxyvitamin D2 and D3
    • Total run time 8.5 min.
    • One sample preparation for all steroid hormones. Easy liquid-liquid extraction step following
      by protein precipitation for the sample treatment-no need for SPE
    • Long life span of HPLC column
  • Parameters

    25-Hydroxy vıtamın D2, 25-Hydroxy vıtamın D3, 3-epı-25-Hydroxy vıtamin D3
  • Matrıx

    • Serum
    • Plasma

Sample Preparation for Serum / Plasma


  • Step 1

    Pipet 200 μL of serum sample into a glass centrifuge tube

  • Step 2

    Add 100 μL of Reagent-1, vortex for 5 sec.

  • Step 3

    Add 25 μL of internal standard solution and additionally vortex for 5 sec.

  • Step 4

    Add 975 μL of Reagent-2 and vortex for 15 sec. Then, centrifuge at 3000 rpm for 5 min.

  • Step 5

    Add 975 μL of Reagent-2 and vortex for 15 sec. Then, centrifuge at 3000 rpm for 5 min.

  • VITAMIN D ANALYSIS RESULT

     

    LOQ (μg/L)

    Recovery (%)

    Repeatability (%)

    25-OH Vitamin D3

    1.52

    93

    2.81

    25-OH Vitamin D2

    3.70

    113

    2.72

  • Extracted chromatogram of 25-OH Vıtamın D2, 25-OH Vıtamın D3, 3-epı-25-OH Vıtamın D3

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..

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