Taurine (2-aminoethanesulphonic acid), a sulphur-containing amino acid, is found in most mammalian tissues. Although it can be synthesized from methionine and cysteine in the presence of vitamin B6, taurine can be obtained from the natural dietary sources. Taurine has many fundamental biological roles which are explained with amino terminal group in the structure and sulfonic acid group moiety.
As public consumer demand for taurine has increased, commercial production of the substance has become necessary, with the introduction of chemical synthesis. Taurine is often included as an ingredient in energy drinks, which is most likely due to its physiological effect to improve muscular function and physical performance.
Jasem method provides straightforward analysis of taurine in energy drinks using LC/MS-MS during 7.5 minutes.
Dilute energy drink sample 10.000 times by using water
Transfer 100 μl of diluted energy drink sample in an HPLC vial
Add 900 μl of Reagent-1 into the vial and vortex for 10 seconds. Then inject to the LC-MS/MS system