Multi-Antibiotics in Animal Feed

Multi-Antibiotics in Animal Feed LC-MSMS Analysis Kit

Antibiotics are widely used in veterinary medicine for prevention of disease in food producing animals. Excessive or unconscious usage of antibiotics might result residue in food such as milk, meat, honey and others. These residues can cause toxicity, allergic reactions. In additions low antibiotics residues in food products, consumed for long time and lead to the spread of drug-resistant bacteria. On the other hand, milk goes through production, processing and circulation until reaching consumers. In order to ensure consumers safety and to prevent any harmful health effects, WHO and EU established the MRL for antibiotics. LC-MS/MS has become the method of choice for analysis antibiotics residues due to do its high sensitivity, selectivity, robustness and cost effective.

Highlights

Analysis of multi-antibiotics in one method
No need SPE or immunoaffinity column for extraction
Quick and simple sample preparation

Parameters

at the bottom of the page

Matrix

Sheep and Cattle feed

Sample Preparation

Step 1

Weight 2.5 g of animal feed into a falcon tube.
Step 2

Add 200 µl of ISTD solution and 10 ml Reagent-1 onto sample and and vortex 5 minutes.
Step 3

Reagent 2 is added to the solution and vortexed for 5 minutes, Centrifuge at 3000 rpm for 5 min.
Step 4

1 ml of the solution is taken, 1 ml of Reagent 3 is added and vortexed for 30 seconds.
Step 5

Filter the clear supernatant from 0.45 µm RC filter into a HPLC vial.

Chromatogram

METHOD PERFORMANCE

Analytes	Linearity (R²) 6460 QQQ	Recovery* (%) 6460 QQQ	Analytes	Linearity (R²) 6460 QQQ	Recovery * (%) 6460 QQQ
5-Hydroxy-Thiabendazole	0.997	98	Metolachlor oxanilic acid	0.994	102
Albendazole	0.993	105	Metronidazole	0.999	92
Albendazole Sulfone	0.999	103	Nalidixic Acid	0.997	109
Albendazole sulfoxide	0.999	108	Oxacillin	0.996	87
Albendazole-2-amine sulfone	0.997	101	Oxytetracycline	0.997	86
Amoxicillin	0.997	102	Oxfendazole	0.997	105
Cloxacillin	0.991	107	Penisilin G	0.997	104
Danofloxacin	0.996	113	Ronidazole	0.999	98
Difloxacin	0.995	96	Sarafloxacin	0.996	93
Dicloxacillin	0.992	114	Sulfadoxine	0.997	104
Dimetridazole	0.995	100	Sulfakloropridazin	0.999	110
Doxycycline	0.993	96	Sulfametaxazole	0.998	106
Enrofloxacin	0.994	101	Sulfamezatin	0.999	107
Fenbendazole	0.993	101	Sulfapyrdine	0.997	101
Flubendazole	0.997	109	Sulfaquinoxaline	0.998	97
Flumacuin	0.993	112	Sulfatiazole	0.998	88
Furaltadon	0.998	91	Sulfadiazine	0.996	92
Ipronidazole	0.995	106	Sulfadimethoxin	0.996	105
Ipronidazole-hyroxy	0.997	114	Sulfamerazine	0.999	102
Chlortetracycline	0.994	98	Tetracycline	0.994	96
Levamisol	0.999	107	Tilmicosin	0.997	118
Marfloxacin	0.995	109	Tylosin	0.999	106
Mebendazole	0.994	118	Triclabendazole	0.998	83

Parameters

5-hyroxy thiobendazole, Albendazole, Albendazole Sulfone, Albendazole sulfoxide, Albendazole-2-amine sulfone, Amoxicillin, Cloxacillin, Danofloxacin, Difloxacin, Dicloxacillin, Dimetridazole, Doxycycline, Enrofloxacin, Fenbendazole, Flubendazole, Flumacuin, Furaltadon, Ipronidazole, Ipronidazole-hyroxy, Chlortetracycline, Levamisol, Marfloxacin, Mebendazole, Metolachlor oxanilic acid, Metronidazole, Nalidixic Acid, Oxacillin, Oxytetracycline, Oxfendazole, Penisilin G, Ronidazole, Sarafloxacin, Sulfadoxine, Sulfakloropridazin, Sulfametaxazole, Sulfamezatin, Sulfapyrdine, Sulfaquinoxaline, Sulfatiazole, Sulfadiazine, Sulfadimethoxin, Sulfamerazine, Tetracycline, Tilmicosin, Tylosin, Triclabendazole