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Patulin in Apple Juice

Patulin in Apple Juice & Apple Puree LC-MS/MS Analysis Kit

Patulin is a mycotoxin mainly found in apples and apple products, and has become one of the most important quality criteria for apple juice.

Maximum permitted concentration has been set for Patulin at 50 µg/L (ppb) in foodstuffs by the World Health Organisation (WHO).

Highlights

Quick and simple sample preparation steps without immunoaffinity column extraction or evaporation.

Parameters

Patulin

Matrix

Apple Juice & Apple Puree

Sample Preparation

For Apple Juice

  • Step 1
    Pipet 1 ml of clear or cloudy apple juice into a glass centrifuge tube
  • Step 2
    Add 3ml of Reagent-1, vortex for 5 seconds
  • Step 3
    Filter mixture from 0.45 µm RC filter, Directly inject into the system.

Sample Preparation

For Apple Puree

  • Step 1
    Weigh 1 g of apple puree into a 50 ml falcon
  • Step 2
    Add 4ml of Reagent-1, vortex for 15 seconds
  • Step 3
    Filter mixture from 0.45 µm RC filter, Directly inject into the system.

Chromatogram

METHOD PERFORMANCE

Matrix

LOQ (ppb) 6460 QQQ

Accuracy (%) 6460 QQQ

Repeatability (%RSD) 6460QQQ

Linearity (R2) 6460 QQQ

Apple juice

10

102.9

4.18

0.998

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..
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