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Aflatoxin M1

Aflatoxin M1 LC-MS/MS Analysis Kit

Aflatoxin M1 (AFM1) is the hydroxylated metabolite of AFB1. For the first time, AFM1 was found in the milk of lactating animals that consume feedstuffs contaminated with AFB1 in the 1960s. For AFM1, the Scientific Committee for Food concluded that there is sufficient evidences that aflatoxin notoxic carcinogen; its carcinogenic potency is estimated to be approximately 10 times lower than aflatoxin B1.

The European Community prescribes that the maximum level of AFM1 in liquid milk should not ex- ceed 0.05 ppb (European Commission 2001). Such low limits require highly sensitive methods for the detection and quantification of AFM1 in milk. Jasem technology focuses on improvement of the sample preparation part, since this analysis-part is till now taking dis-proportional long time in exist- ing reference & in house methods.

Highlights

No need for immunoaffinity column extraction or or evaporation
Quick and simple sample preparation.
7.5 min. analysis time.

Parameters

Aflatoxin M1

Matrix

Milk, milk powder, infant powder, infant milk powder

Sample Preparation


  • Step 1
    Weigh 1 g of infant powder into a 50 ml polypropylene centrifuge tube
  • Step 2
    Add 10 ml of water and dissolve the powder by vortexing.
  • Step 3
    Pipet 1 ml from the 10 ml milk powder solution into a glass centrifuge tube.
  • Step 4
    Add 4 ml of Reagent 1 into the centrifuge tube and vortex for 15 seconds.
  • Step 5
    Centrifuge at 3000 rpm for 4 minutes,filter supernatant from 0.45 µm nylon filter into a HPLC vial.

Chromatogram

METHOD PERFORMANCE

Matrix

LOQ

6460

(µg/L)

Accuracy

6460

(%)

Repeatability

6460

(%RSD)

Linearity

6460

(R2)

Goat Milk

0.017

117

3.04

0.995

Infant Milk

0.015

108

3.8

0.996

Infant Powder

0.013

108

6.0

0.996

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..
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