
Aflatoxin M1 LC-MS/MS Analysis Kit
Aflatoxin M1 (AFM1) is the hydroxylated metabolite of AFB1. For the first time, AFM1 was found in the milk of lactating animals that consume feedstuffs contaminated with AFB1 in the 1960s. For AFM1, the Scientific Committee for Food concluded that there is sufficient evidences that aflatoxin notoxic carcinogen; its carcinogenic potency is estimated to be approximately 10 times lower than aflatoxin B1.
The European Community prescribes that the maximum level of AFM1 in liquid milk should not ex- ceed 0.05 ppb (European Commission 2001). Such low limits require highly sensitive methods for the detection and quantification of AFM1 in milk. Jasem technology focuses on improvement of the sample preparation part, since this analysis-part is till now taking dis-proportional long time in exist- ing reference & in house methods.
Highlights
Quick and simple sample preparation.
7.5 min. analysis time.
Parameters
Matrix
Sample Preparation
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Step 1Weigh 1 g of infant powder into a 50 ml polypropylene centrifuge tube
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Step 2Add 10 ml of water and dissolve the powder by vortexing.
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Step 3Pipet 1 ml from the 10 ml milk powder solution into a glass centrifuge tube.
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Step 4Add 4 ml of Reagent 1 into the centrifuge tube and vortex for 15 seconds.
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Step 5Centrifuge at 3000 rpm for 4 minutes,filter supernatant from 0.45 µm nylon filter into a HPLC vial.

Chromatogram
METHOD PERFORMANCE
Matrix | LOQ 6460 (µg/L) | Accuracy 6460 (%) | Repeatability 6460 (%RSD) | Linearity 6460 (R2) |
Goat Milk | 0.017 | 117 | 3.04 | 0.995 |
Infant Milk | 0.015 | 108 | 3.8 | 0.996 |
Infant Powder | 0.013 | 108 | 6.0 | 0.996 |