Multi-Antibiotics in Milk and Yoghurt LC-MS/MS Analysis Kit
Antibiotics are widely used in veterinary medicine for prevention of disease in food producing animals. Excessive or unconscious usage of antibiotics might result residue in food such as milk, meat, honey and others. These residues can cause toxicity, allergic reactions. In additions low antibiotics residues in food products, consumed for long time and lead to the spread of drug-resistant bacteria. On the other hand, milk goes through production, processing and circulation until reaching consumers. In order to ensure consumers safety and to prevent any harmful health effects, WHO and EU established the MRL for antibiotics. LC-MS/MS has become the method of choice for analysis antibiotics residues due to do its high sensitivity, selectivity, robustness and cost effective.
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Highlights
Analysis of multi-antibiotics in one method
No need SPE or immunoaffinity column for extraction
Quick and simple sample preparation -
Parameters
at the bottom of the page -
Matrix
Milk & Yoghurt
Sample Preparation
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Step 1Pipet 1 ml of milk or weight 1.0 g of yoghurt sample into a centrifuge tube.
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Step 2Add 60 µl of ISTD solution and 2.0 ml Reagent-1 onto sample and vortex for 30 second.
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Step 3Centrifuge at 3000 rpm for 10 min.
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Step 4Filter the clear supernatant from 0.45 µm RC filter into a HPLC vial.
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Chromatogram
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METHOD PERFORMANCE
Analytes
LOQ (µg/L) 6460 QQQ
Accuracy (%) 6460 QQQ
Repeatability (%RSD) 6460 QQQ
Linearity (R2) 6460 QQQ
5-hyroxy thiobendazole
0.47
99
1.49
0.996
Abamectin
0.84
101
9.39
0.999
Albendazole
0.12
106
1.59
0.992
Ampicillin
0.78
101
5.5
0.995
Amoxicillin
0.33
102
1.91
0.998
Ciprofloxacin
0.4
100
1.54
0.997
Danofloxacin
0.26
97
0.94
0.997
Difloxacin
0.25
106
1.98
0.998
Doxycycline
0.51
64
7.08
0.996
Doramectin
0.95
108
4.74
0.993
Enrofloxacin
0.29
103
1.21
0.991
Epinomectin
0.55
98
8.03
0.995
Erythromycin
0.45
104
4.14
0.996
Fenbendazole
0.1
107
1.74
0.997
Flumequine
0.12
104
2.03
0.998
Ipronidazole
0.68
97
1.75
0.996
Ipronidazole-hydroxy
1.06
88
7.84
0.992
Ivermectin
0.58
98
5.91
0.996
Chlortetracycline
4.29
102
5.26
0.996
Levamisole HCl
0.22
115
1.4
0.999
Marbofloxacin
0.15
110
1.19
0.999
Mebendazole
0.28
103
1.87
0.998
Metronidazole
0.78
97
2.28
0.993
Moxidectin
0.55
96
4.52
0.995
Nalidixic acid
0.16
108
2.19
0.997
Naracin
0.09
102
4.59
0.996
Oxacillin
3.67
108
4.96
0.999
Oxytetracycline
0.86
109
4.73
0.991
Oxfendazole
0.34
98
1.42
0.993
Oxolinic acid
0.16
112
1.58
0.996
Penicillin G
0.32
102
1.93
0.992
Ronidazole
0.96
98
2.52
0.999
Salinomcysin
0.1
102
7.25
0.995
Sarafloxacin
0.52
97
2.09
0.993
Sulfadiazin
0.32
95
3.08
0.992
Sulfadimethoxin
0.56
101
1.58
0.998
Sulfadioxin
0.29
100
2.06
0.996
Analytes
LOQ (µg/L) 6460 QQQ
Accuracy (%) 6460 QQQ
Repeatability (%RSD) 6460 QQQ
Linearity (R2) 6460 QQQ
Sulfachloropyridazine
1.48
89
6.12
0.997
Sulfamerazin
0.44
101
1.36
0.998
Sulfametoxazole
0.95
73
3.7
0.996
Sulfamethazine
0.54
97
1.96
0.994
Sulfathiazole
0.79
96
1.65
0.992
Sulfapyrdine
0.39
112
2.48
0.995
Sulfaquinoxaline
0.58
104
2.24
0.994
Tetracycline
1.04
98
5.3
0.997
Thiabendazole
0.34
86
3.5
0.998
Tilmicosin
0.48
111
1.7
0.995
Tylosin
0.7
107
1.71
0.996
Triclabendazole
0.83
108
4.43
0.992
Toltrazural
0.11
102
4.08
0.995
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5-hyroxy thiobendazole, Abamectin, Albendazole, Ampicillin, Amoxicillin, Ciprofloxacin, Danofloxacin, Difloxacin, Doxycycline, Doramectin, Enrofloxacin, Epinomectin, Erythromycin, Fenbendazole, Flumequine, Ipronidazole, Ipronidazole-hydroxy, Ivermectin, Chlortetracycline, Levamisole, Marbofloxacin, Mebendazole, Metronidazole, Moxidectin, Nalidixic acid, Naracin, Oxacillin, Oxytetracycline, Oxfendazole, Oxolinic acid, Sulfadiazin, Sulfadimethoxin, Sulfadioxin, Sulfachloropyridazine, Sulfamerazin, Sulfametoxazole, Sulfamethazine, Sulfathiazole, Sulfapyrdine, Sulfaquinoxaline, Tetracycline, Penicillin G, Ronidazole, Salinomcysin, Sarafloxacin, Thiabendazole, Tilmicosin, Tylosin, Triclabendazole, Toltrazural