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Multi-Antibiotics

Multi-Antibiotics in Milk and Yoghurt LC-MS/MS Analysis Kit

Antibiotics are widely used in veterinary medicine for prevention of disease in food producing animals. Excessive or unconscious usage of antibiotics might result residue in food such as milk, meat, honey and others. These residues can cause toxicity, allergic reactions. In additions low antibiotics residues in food products, consumed for long time and lead to the spread of drug-resistant bacteria. On the other hand, milk goes through production, processing and circulation until reaching consumers. In order to ensure consumers safety and to prevent any harmful health effects, WHO and EU established the MRL for antibiotics. LC-MS/MS has become the method of choice for analysis antibiotics residues due to do its high sensitivity, selectivity, robustness and cost effective.

  • Highlights

    Analysis of multi-antibiotics in one method

    No need SPE or immunoaffinity column for extraction

    Quick and simple sample preparation
  • Parameters

    at the bottom of the page
  • Matrix

    Milk & Yoghurt

Sample Preparation


  • Step 1
    Pipet 1 ml of milk or weight 1.0 g of yoghurt sample into a centrifuge tube.
  • Step 2
    Add 60 µl of ISTD solution and 2.0 ml Reagent-1 onto sample and vortex for 30 second.
  • Step 3
    Centrifuge at 3000 rpm for 10 min.
  • Step 4
    Filter the clear supernatant from 0.45 µm RC filter into a HPLC vial.
  • Chromatogram

  • METHOD PERFORMANCE

    Analytes

    LOQ (µg/L) 6460 QQQ

    Accuracy (%) 6460 QQQ

    Repeatability (%RSD) 6460 QQQ

    Linearity (R2) 6460 QQQ

    5-hyroxy thiobendazole

    0.47

    99

    1.49

    0.996

    Abamectin

    0.84

    101

    9.39

    0.999

    Albendazole

    0.12

    106

    1.59

    0.992

    Ampicillin

    0.78

    101

    5.5

    0.995

    Amoxicillin

    0.33

    102

    1.91

    0.998

    Ciprofloxacin

    0.4

    100

    1.54

    0.997

    Danofloxacin

    0.26

    97

    0.94

    0.997

    Difloxacin

    0.25

    106

    1.98

    0.998

    Doxycycline

    0.51

    64

    7.08

    0.996

    Doramectin

    0.95

    108

    4.74

    0.993

    Enrofloxacin

    0.29

    103

    1.21

    0.991

    Epinomectin

    0.55

    98

    8.03

    0.995

    Erythromycin

    0.45

    104

    4.14

    0.996

    Fenbendazole

    0.1

    107

    1.74

    0.997

    Flumequine

    0.12

    104

    2.03

    0.998

    Ipronidazole

    0.68

    97

    1.75

    0.996

    Ipronidazole-hydroxy

    1.06

    88

    7.84

    0.992

    Ivermectin

    0.58

    98

    5.91

    0.996

    Chlortetracycline

    4.29

    102

    5.26

    0.996

    Levamisole HCl

    0.22

    115

    1.4

    0.999

    Marbofloxacin

    0.15

    110

    1.19

    0.999

    Mebendazole

    0.28

    103

    1.87

    0.998

    Metronidazole

    0.78

    97

    2.28

    0.993

    Moxidectin

    0.55

    96

    4.52

    0.995

    Nalidixic acid

    0.16

    108

    2.19

    0.997

    Naracin

    0.09

    102

    4.59

    0.996

    Oxacillin

    3.67

    108

    4.96

    0.999

    Oxytetracycline

    0.86

    109

    4.73

    0.991

    Oxfendazole

    0.34

    98

    1.42

    0.993

    Oxolinic acid

    0.16

    112

    1.58

    0.996

    Penicillin G

    0.32

    102

    1.93

    0.992

    Ronidazole

    0.96

    98

    2.52

    0.999

    Salinomcysin

    0.1

    102

    7.25

    0.995

    Sarafloxacin

    0.52

    97

    2.09

    0.993

    Sulfadiazin

    0.32

    95

    3.08

    0.992

    Sulfadimethoxin

    0.56

    101

    1.58

    0.998

    Sulfadioxin

    0.29

    100

    2.06

    0.996

    Analytes

    LOQ (µg/L) 6460 QQQ

    Accuracy (%) 6460 QQQ

    Repeatability (%RSD) 6460 QQQ

    Linearity (R2) 6460 QQQ

    Sulfachloropyridazine

    1.48

    89

    6.12

    0.997

    Sulfamerazin

    0.44

    101

    1.36

    0.998

    Sulfametoxazole

    0.95

    73

    3.7

    0.996

    Sulfamethazine

    0.54

    97

    1.96

    0.994

    Sulfathiazole

    0.79

    96

    1.65

    0.992

    Sulfapyrdine

    0.39

    112

    2.48

    0.995

    Sulfaquinoxaline

    0.58

    104

    2.24

    0.994

    Tetracycline

    1.04

    98

    5.3

    0.997

    Thiabendazole

    0.34

    86

    3.5

    0.998

    Tilmicosin

    0.48

    111

    1.7

    0.995

    Tylosin

    0.7

    107

    1.71

    0.996

    Triclabendazole

    0.83

    108

    4.43

    0.992

    Toltrazural

    0.11

    102

    4.08

    0.995

  • 5-hyroxy thiobendazole, Abamectin, Albendazole, Ampicillin, Amoxicillin, Ciprofloxacin, Danofloxacin, Difloxacin, Doxycycline, Doramectin, Enrofloxacin, Epinomectin, Erythromycin, Fenbendazole, Flumequine, Ipronidazole, Ipronidazole-hydroxy, Ivermectin, Chlortetracycline, Levamisole, Marbofloxacin, Mebendazole, Metronidazole, Moxidectin, Nalidixic acid, Naracin, Oxacillin, Oxytetracycline, Oxfendazole, Oxolinic acid, Sulfadiazin, Sulfadimethoxin, Sulfadioxin, Sulfachloropyridazine, Sulfamerazin, Sulfametoxazole, Sulfamethazine, Sulfathiazole, Sulfapyrdine, Sulfaquinoxaline, Tetracycline, Penicillin G, Ronidazole, Salinomcysin, Sarafloxacin, Thiabendazole, Tilmicosin, Tylosin, Triclabendazole, Toltrazural
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