Multi-Mycotoxin In Baby

Multi-Mycotoxin In Baby LC-MS/MS Analysis Kit

Mycotoxins are secondary metabolites produced by molds, without biochemical significance in fungal growth and development. Although they have existed since ancient times, the term mycotoxins appeared in 1960 when 100 000 turkey poults died due to the ingestion of peanut contaminated with secondary metabolites from Aspergillus flavus.

Mycotoxins are compounds with various chemical structures and, therefore, various biological effects in animals and humans such as toxic, carcinogenic, estrogenic, mutagenic, teratogenic, and immunotoxic effects.

In Europe, Commission Regulation (EC) No 1881/2006 and its amendments set maximum residue levels (MRLs) for 13 mycotoxins.

Jasem multi-mycotoxin method analyzes 10 mycotoxins in one injection and HT-2 and DON are analyzed in second injection with same sample, same column and same instrument parameters except HPLC program.

Highlights

Quick and simple sample preparation steps without immunoaffinity column extraction or evaporation

Parameters

AF B1, AF B2, AF G1, AF G2, FB1, FB2, OTA, DON, ZON, HT2, T2, STE

Matrix

Baby Food (Cereal and or Milk-based)

Sample Preparation

Step 1

Weigh 2 g of fine blended baby food matrix (cereal or milk based) into a 50 ml polypropylene falcon.
Step 2

Add 10 ml Reagent 1 onto sample and shake for 15 minutes using multiple-shaker.
Step 3

Centrifuge at 3000 rpm for 5 min.
Step 4

Filter the clear supernatant from 0.45 µm nylon filter into a HPLC vial.

Chromatogram

METHOD PERFORMANCE

Analytes	LOQ*(µg/L) 6460 QQQ	Accuracy (%) 6460 QQQ	Repeatability (%RSD) 6460 QQQ	Linearity (R²) 6460 QQQ
AFB1	0.05	101.24	1.17	0.998
AFB2	0.20	101.47	2.05	0.997
AFG1	0.27	95.55	3.33	0.997
AFG2	0.28	92.43	3.35	0.998
DON	21.93	101.87	4.09	0.997
FB1	5.46	89.9	3.3	0.998
FB2	5.50	94.6	2.38	0.998
HT2	4.74	98.94	2.53	0.997
OTA	0.10	94.52	3.9	0.997
STE	0.13	96.04	2.56	0.999
T2	1.09	96.05	4.85	0.998
ZON	1.10	110.65	1.37	0.997

* LOQ: Limit of quantification, 10sd + AVERAGE