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Taurine in Energy Drinks

Taurine in Energy Drinks LC-MS/MS Analysis Kit

Taurine (2-aminoethanesulphonic acid), a sulphur-containing amino acid, is found in most mammalian tissues. Although it can be synthesized from methionine and cysteine in the presence of vitamin B6, taurine can be obtained from the natural dietary sources. Taurine has many fundamental biological roles which are explained with amino terminal group in the structure and sulfonic acid group moiety.

As public consumer demand for taurine has increased, commercial production of the substance has become necessary, with the introduction of chemical synthesis. Taurine is often included as an ingredient in energy drinks, which is most likely due to its physiological effect to improve muscular function and physical performance.

Jasem method provides straightforward analysis of taurine in energy drinks using LC/MS-MS Total run time is 7.5 minutes.

Highlights

Easy sample preparation without the need for solid phase extraction (SPE) and additional concentration steps.
Dilute and shoot samples

Parameters

Taurine

Matrix

Energy Drinks

Sample Preparation


  • Step 1
    Dilute energy drink sample 10000 times by using water.
  • Step 2
    Transfer 100 µl of diluted energy drink sample in an HPLC vial.
  • Step 3
    Add 900 µl of Reagent-1 into the sample vial and vortex 5 secend.
  • Step 4
    Filter out from 0.45 µm nylon filters, directly inject into the system.

Chromatogram

METHOD PERFORMANCE

Analytes

LOQ (ppb) 6460 QQQ

Accuracy (%) 6460 QQQ

Repeatability (%RSD) 6460 QQQ

Linearity (R2) 6460QQQ

Taurine

10

98

1.25

0.999

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..
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