
HVA-VMA-5HIAA in Urine
HVA-VMA-5HIAA in Urine LC-MS/MS Analysis Kit
Laboratory measurement of vanillylmandelic acid (VMA), the product of epinephrine, norepinephrine metabolism, and homovanillic acid (HVA), a terminal metabolite of dopamine, and 5-Hydroxyindolacetic acid (5-HIAA) the end metabolite of serotonin is used to enhance clinical diagnosis and management of catecholamine-secreting neurochromaffin tumors such as neuroblastomas. Neuroblastoma usually emerges in children and accounts for 7–10% of childhood cancer; approximately 90% of these tumors produce catecholamines (CAs). Pheochromocytoma is a rare neuroendocrine tumor arising from the chromaffin cells of the adrenal medulla and characterized by producing excessive amounts of CAs and their metabolites.
Highlights
Simultaneously quantification of HVA, VMA and 5-HIAA
Dilute and shoot sample preparation procedure
Total run time 10 min.
Dilute and shoot sample preparation procedure
Total run time 10 min.
Parameters
Homovanillic acid
Vanillylmandelic acid
5-Hydroxyindolacetic acid
Vanillylmandelic acid
5-Hydroxyindolacetic acid
Matrix
Urine
Sample Preparation
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Step 1Pipette 50 µL of calibrator/control/urine sample into a sample via
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Step 2Add 50 µL of IS solution, vortex for 5 sec
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Step 3Add 400 µL of Reagent-1 addition to vortex for 5 sec. prior to injection

Chromatogram
METHOD PERFORMANCE
Analyte | LOQ (mg/L) 6460 QQQ | LOQ (mg/L) Ultivo QQQ | LOQ (mg/L) 6470 QQQ | Accuracy % 6470 QQQ | Repeatabilty % CV 6470 | Linearity R2 6470 QQQ |
Homovanillic Acid | 1,769 | 0,622 | 2,75 | 117,41 | 3,23 | 0,997 |
Vanillylmandelic Acid | 1,972 | 0,025 | 3,99 | 107,74 | 3,9 | 0,998 |
5-Hydroxyindolacetic Acid | 1,212 | 0,037 | 1,97 | 81,4 | 6,45 | 0,999 |