Multi-Antibiotics In Honey

Multi-Antibiotics In Honey LC-MS/MS Analysis Kit

Antibiotics are widely used in veterinary medicine for prevention of disease in food producing animals. Excessive or unconscious usage of antibiotics might result residue in food such as milk, meat, honey and others. These residues can cause toxicity, allergic reactions. In additions low antibiotics residues in food products, consumed for long time and lead to the spread of drug-resistant bacteria. On the other hand, milk goes through production, processing and circulation until reaching consumers. In order to ensure consumers’ safety and to prevent any harmful health effects, WHO and EU established the MRL for antibiotics. LC-MS/MS has become the method of choice for analysis antibiotics residues due to do its high sensitivity, selectivity, robustness and cost effective.

Highlights

Analysis of multi-antibiotics in one method

No need SPE or immunoaffinity column for extraction

Quick and simple sample preparation

Parameters

at the bottom of the page

Matrix

Honey

Sample Preparation

Step 1

5 g of honey sample is weighed into the plastic centrifuge tube.
Step 2

Add 200 µl ISTD solution, 20 ml Reagent-1 on the sample and shake for 15 minutes.
Step 3

Centrifuge at 3000 rpm for 10 min.
Step 4

Filter the clear supernatant from 0.45 µm PTFE filter into a HPLC vial.

Chromatogram

METHOD PERFORMANCE

Analytes	LOQ (µg/L) ULTIVO QQQ	Accuracy (%) ULTIVO QQQ	Repeatability (%RSD) ULTIVO QQQ	Linearity (R²) ULTIVO QQQ
Albendazole	0.89	92	0.45	0.999
Chloramphenicol	2.45	92	1.39	0.995
Chlortetracycline	2.79	98	1.28	0.993
Ciprofloxacin	3.10	80	2.65	0.992
Dihydrostreptomycin	0.42	70	0.20	0.998
Doxycycline	3.08	83	1.77	0.996
Enrofloxacin	1.48	90	0.96	0.997
Erythromycin	1.78	78	0.97	0.998
Fenbendazole	0.87	84	0.43	0.996
Flubendazole	0.94	100	0.48	0.994
Furazolidon	1.24	81	0.64	0.992
Levamisole	0.63	87	0.33	0.995
marbofloxacin	2.49	85	1.25	0.996
Mebendazole	1.36	95	0.68	0.997
Oxolinic acid	0.77	93	0.42	0.998
Oxytetracycline	3.11	100	1.71	0.996
Penicillin G	2.50	94	1.85	0.992
Streptomycin	2.10	104	0.84	0.996
Sulfachloropyridazine	1.64	88	0.84	0.996
Sulfadiazine (Silvadene)	0.59	85	0.30	0.999
Sulfadimethoxine	0.86	90	0.46	0.999
Sulfadimidine(Sulfamethazine)	1.56	85	0.78	0.998
Sulfadoxine	0.86	91	0.46	0.993
Sulfamerazine	1.18	89	0.55	0.995
Sulfamethoxazole	1.22	91	0.60	0.997
Sulfapyridine	2.43	83	1.30	0.996
Sulfaquinoxaline	0.92	96	0.48	0.999
Sulfathiazole	2.14	81	1.19	0.991
Tetracycline	3.22	100	2.08	0.993
Thiabendazole	0.71	91	0.40	0.996
Tylosin	2.17	76	1.31	0.992

Parameters

Albendazole, Chloramphenicol, Chlortetracycline, Ciprofloxacin, Dihydrostreptomycin, Doxycycline, Enrofloxacin, Erythromycin, Fenbendazole, Flubendazole, Furazolidon, Levamisole, marbofloxacin, Mebendazole, Oxolinic acid, Oxolinic acid, Oxytetracycline, Penicillin G, Sulfadoxine, Sulfamerazine, Sulfamethoxazole, Sulfapyridine, Sulfaquinoxaline, Sulfathiazole, Sulfaquinoxaline, Sulfathiazole, Sulfachloropyridazine, Sulfadiazine (Silvadene), Sulfadimethoxine, Sulfadimidine (Sulfamethazine), Streptomycin, Tetracycline, Thiabendazole, Tylosin.