
NEONATAL SCREENING
Newborn Screening in DBS LC-MS/MS Analysis Kit
Newborn screening (NBS) is the blood test (frequently collecting blood specimen by heel prick) which newborn infants are screened just after nativity for a list of diseases that can evoke life threatening and/or intellectual disability unless uncovered and treated. Screening programs are run with the goal of preventing or diminishing genetic, metabolic, hormonal, and functional disorders through appropriate referral and early treatment.
Highlights
Analysis from DBS extract without derivatization.
Quick sample preparation-just pipette and agitate at room temperature.
2-minute simultaneous analysis of 14 amino acids and 37 free carnitine/acylcarnitines.
Quick sample preparation-just pipette and agitate at room temperature.
2-minute simultaneous analysis of 14 amino acids and 37 free carnitine/acylcarnitines.
Parameters
Alanine, Arginine, Argininosuccinic Acid, Citrulline, Glutamic Acid, Glycin, Leucine, Methionine, Ornithine, Phenylalanine, Proline, Tyrosine, Valine
C0, C2, C3, C3DC + C4-OH, C4, C4DC + C5-OH, C5, C5DC, C5:1, C6, C6:1, C6-OH, C8, C8DC, C8:1, C10, C10DC, C10:1, C10:1-OH, C10:2, C12, C12DC, C12:1, C12:1-OH, C14, C14:1, C14:2, C16, C16:1, C16:1-OH, C16-OH, C18, C18:1, C18:1-OH, C18:2, C18-OH
C0, C2, C3, C3DC + C4-OH, C4, C4DC + C5-OH, C5, C5DC, C5:1, C6, C6:1, C6-OH, C8, C8DC, C8:1, C10, C10DC, C10:1, C10:1-OH, C10:2, C12, C12DC, C12:1, C12:1-OH, C14, C14:1, C14:2, C16, C16:1, C16:1-OH, C16-OH, C18, C18:1, C18:1-OH, C18:2, C18-OH
Matrix
Dried Blood Spot (DBS)
Sample Preparation
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Step 1Punch 3 mm of dried blood spot sample/control into an eppendorf tube.
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Step 2Add 100 µl of IS mixture (for extraction).
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Step 3Gently shake for 15 minutes at room temperature.
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Step 4Pipette the supernatant into an insert-fitted HPLC vial prior to injection.
