
Sulfonamides In Honey LC-MS/MS Analysis Kit
Sulfonamides belong to a large and important class of antibacterial drugs and are very common in veterinary and human medicine.
Honey bee larvae are susceptible to American foulbrood (AFB) or European Foulbrood (EFB), disease caused by the organism Streptococcus pluton bacteria and Paenibacilus (Bacillus) larvae, respectively, which can devastate hives.
Sulfonamides are relatively stable chemotherapeutics known to control this disease but they are not permitted to use for this purpose in most countries because of the potential of sulfonamide residues to contaminate honey.
Highlights
Parameters
Matrix
Sample Preparation
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Step 1Accurately transfer 1 g of honey sample by weighing into a glass experiment tube.
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Step 2Add 1 ml of Reagent 1 and vortex it until dissolved completely about 15 seconds.
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Step 3Stand sample at 50 °C in water bath for 15 minutes.
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Step 4Add 3 ml of Reagent 2 onto honey sample and vortex 15 seconds.
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Step 5Filter out from 0.45 µm RC filters, Directly inject into the system.

Chromatogram
METHOD PERFORMANCE
Analytes | LOQ (ppb) 6460 QQQ | Accuracy (%) 6460 QQQ | Repeatability (%RSD) 6460 QQQ | Linearity (R2) 6460 QQQ |
Sulfadiazin | 6.80 | 98.99 | 1.58 | 0.999 |
Sulfathiazole | 6.51 | 94.66 | 3.24 | 0.998 |
Sulfapyridine | 6.79 | 94.58 | 2.24 | 0.999 |
Sulfamerazine | 7.34 | 95.16 | 1.34 | 0.998 |
Sulfamethazine | 6.06 | 94.93 | 1.30 | 0.998 |
Sulfachloropyridazine | 7.94 | 105.4 | 2.54 | 0.995 |
Sulfadoxin | 6.57 | 91.19 | 2.02 | 0.997 |
Sulfamethoxazole | 7.93 | 89.24 | 3.64 | 0.996 |
Sulfaquinoxaline | 5.60 | 97.05 | 3.35 | 0.995 |
Sulfadimethoxine | 6.90 | 96.50 | 1.95 | 0.997 |