Sulfonamides In Honey

Sulfonamides In Honey LC-MS/MS Analysis Kit

Sulfonamides belong to a large and important class of antibacterial drugs and are very common in veterinary and human medicine.

Honey bee larvae are susceptible to American foulbrood (AFB) or European Foulbrood (EFB), disease caused by the organism Streptococcus pluton bacteria and Paenibacilus (Bacillus) larvae, respectively, which can devastate hives.

Sulfonamides are relatively stable chemotherapeutics known to control this disease but they are not permitted to use for this purpose in most countries because of the potential of sulfonamide residues to contaminate honey.

Highlights

Easy sample preparation without the need for solid phase extraction (SPE) and additional concentration steps.

Parameters

at the bottom of the page

Matrix

Honey

Sample Preparation

Step 1

Accurately transfer 1 g of honey sample by weighing into a glass experiment tube.
Step 2

Add 1 ml of Reagent 1 and vortex it until dissolved completely about 15 seconds.
Step 3

Stand sample at 50 °C in water bath for 15 minutes.
Step 4

Add 3 ml of Reagent 2 onto honey sample and vortex 15 seconds.
Step 5

Filter out from 0.45 µm RC filters, Directly inject into the system.

Chromatogram

METHOD PERFORMANCE

Analytes	LOQ (ppb) 6460 QQQ	Accuracy (%) 6460 QQQ	Repeatability (%RSD) 6460 QQQ	Linearity (R²) 6460 QQQ
Sulfadiazin	6.80	98.99	1.58	0.999
Sulfathiazole	6.51	94.66	3.24	0.998
Sulfapyridine	6.79	94.58	2.24	0.999
Sulfamerazine	7.34	95.16	1.34	0.998
Sulfamethazine	6.06	94.93	1.30	0.998
Sulfachloropyridazine	7.94	105.4	2.54	0.995
Sulfadoxin	6.57	91.19	2.02	0.997
Sulfamethoxazole	7.93	89.24	3.64	0.996
Sulfaquinoxaline	5.60	97.05	3.35	0.995
Sulfadimethoxine	6.90	96.50	1.95	0.997

Parameters

Sulfadiazin,Sulfathiazole,Sulfapyridine,Sulfamerazine,Sulfamethazine,Sulfachloropyridazine,Sulfadoxin, Sulfamethoxazole, Sulfaquinoxaline, Sulfadimethoxine