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Tetracyclines In Milk

Tetracyclines In Milk LC-MS/MS Analysis Kit

Antibiotics are used worldwide to control bacterial infection and promote healthy farm animals for milk production. Tetracyclines (TCs) are broad-spectrum antibiotics, widely used in animal husbandry.

In cattle, tetracyclines are used when treating general, respiratory, urinary, and local infections and frequent milk contamination is intramammary administration.

Milk tetracyclines contents reach 50-60% concentrations of those in blood plasma. To prevent any harmful health effects such as permanent stained teeth, hepatoxicity, hypersensitivity reactions on consumers, WHO and EU established the MRL for veterinary drugs.

Highlights

Easy sample preparation without the need for solid phase extraction (SPE) and additional concentration steps.

Parameters

Chlortetracycline, Oxytetracycline, Tetracycline, Doxycycline

Matrix

Milk

Sample Preparation


  • Step 1
    Accurately transfer by pipetting 4 ml of milk sample into a glass experiment tube.
  • Step 2
    Add 1 ml of Reagent 1 and vortex 30 seconds vigorously.
  • Step 3
    Centrifuge at 3000 rpm for 5 minutes.
  • Step 4
    Filter out from 0.45 µm RC filters,directly inject into the system.

Chromatogram

METHOD PERFORMANCE

Analytes

LOQ (ppb) 6460 QQQ

Accuracy (%) 6460 QQQ

Repeatability (%RSD) 6460 QQQ

Linearity (R2) 6460 QQQ

Chlortetracycline

1.01

82.09

3.94

0.9999

Oxytetracycline

1.06

84.47

2.90

0.9999

Tetracycline

1.08

103.16

4.20

0.9996

Doxycycline

1.00

112.64

3.98

0.9999

* LOQ: Limit of quantification, 10sd + AVERAGE

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..
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