
Tetracyclines In Milk LC-MS/MS Analysis Kit
Antibiotics are used worldwide to control bacterial infection and promote healthy farm animals for milk production. Tetracyclines (TCs) are broad-spectrum antibiotics, widely used in animal husbandry.
In cattle, tetracyclines are used when treating general, respiratory, urinary, and local infections and frequent milk contamination is intramammary administration.
Milk tetracyclines contents reach 50-60% concentrations of those in blood plasma. To prevent any harmful health effects such as permanent stained teeth, hepatoxicity, hypersensitivity reactions on consumers, WHO and EU established the MRL for veterinary drugs.
Highlights
Parameters
Matrix
Sample Preparation
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Step 1Accurately transfer by pipetting 4 ml of milk sample into a glass experiment tube.
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Step 2Add 1 ml of Reagent 1 and vortex 30 seconds vigorously.
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Step 3Centrifuge at 3000 rpm for 5 minutes.
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Step 4Filter out from 0.45 µm RC filters,directly inject into the system.

Chromatogram
METHOD PERFORMANCE
Analytes | LOQ (ppb) 6460 QQQ | Accuracy (%) 6460 QQQ | Repeatability (%RSD) 6460 QQQ | Linearity (R2) 6460 QQQ |
Chlortetracycline | 1.01 | 82.09 | 3.94 | 0.9999 |
Oxytetracycline | 1.06 | 84.47 | 2.90 | 0.9999 |
Tetracycline | 1.08 | 103.16 | 4.20 | 0.9996 |
Doxycycline | 1.00 | 112.64 | 3.98 | 0.9999 |
* LOQ: Limit of quantification, 10sd + AVERAGE