Tetracyclines In Tissue

Tetracyclines In Tissue LC-MS/MS Analysis Kit

Tetracyclines (TCs) are broad-spectrum antibiotics, widely used in animal husbandry for prevention and treatment of diseases and as growth promotion in food-producing animals.

Tetracyclines can be administrated orally through food or drinking water, parenterally, or by intra-mammary infusion.

Tetracyclines in meat potentially may stain teeth of young children. Allergic reactions and development of resistant strains of bacteria following the ingestion of subtherapeutic doses of antimicrobials are some of the hazardous effects.

Highlights

Easy sample preparation without the need for solid phase extraction (SPE) and additional concentration steps.

Parameters

Chlortetracycline, Oxytetracycline, Tetracycline, Doxycycline

Matrix

Poultry and Red meat tissues

Sample Preparation

Step 1

Weigh 5 g of minced meat sample into a 50 ml polypropylene falcon
Step 2

Add 100 µl of 5 ppm ISTD mix onto meat sample and vortex 10 seconds.
Step 3

Add 5 ml of Reagent 2 and vortex additional 1 minutes.
Step 4

Centrifuge at 3000 rpm for 5 minutes.
Step 5

Filter out from 0.45 µm RC filters,Directly inject into the system.

Chromatogram

METHOD PERFORMANCE

Analytes	LOQ* (ppb) 6460 QQQ	Accuracy (%) 6460 QQQ	Repeatability (%RSD) 6460 QQQ	Linearity (R²) 6460 QQQ
Chlortetracycline	14.42	91.74	4.32	0.998
Oxytetracycline	15.01	95.45	2.80	0.997
Tetracycline	15.14	91.27	2.42	0.997
Doxycycline	15.46	92.24	2.59	0.997