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Trimethylamine-N-Oxide(TMAO) in Serum/Plasma LC-MS/MS Analysis Kit

The human gut contains a complex and metabolically active microbial ecosystem (microbiota). The gut microbiome has recently been proven to play an important role in cardiovascular disease and various other disorders. Among the microbiome-related metabolites, trimethylamine-N-oxide (TMAO) has drawn a lot of attention owing to its proatherogenic and prothrombotic properties. Thus, abnormality in circulating level of TMAO could be a risk factor for certain diseases.

Highlights

Quantitative analysis of TMAO in 5 min. for serum/plasma.

Just a few pipetting steps for the sample preparation; for plasma/serum-protein crash and shoot.

Safeguarded by its own stable labelled isotope (TMAO-D9) as internal standard.

Consuming small volume of patient’s sample.

Long life span of HPLC column.

Parameters

Trimethylamin-N-Oxide (TMAO)

Matrix

Serum/Plasma

Sample Preparation

  • Step 1
    Pipette 50 μL of serum/plasma sample into a glass centrifuge tube
  • Step 2
    Add 25 μL of internal standard solution and vortex for 5 sec.
  • Step 3
    Add 425 μL of reagent-1 (for protein precipitation), vortex for 5 sec. then, centrifuge at 4000 rpm for 5 min.
  • Step 4
    Decant the clear supernatant into a HPLC vial prior to injection

Chromatogram

Method Performance

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..
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