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Antiepileptic Drugs in Serum/Plasma

Antiepileptic Drugs in Serum/Plasma LC-MS/MS Analysis Kit

Epilepsy is defined as seizure disorder induced by surge of electrical activity in the brain. Anticonvulsant or antiepileptic medications encompass a broad range of drugs that are primarily intended to control ongoing or anticipated future seizures as well as being beneficial in non-epileptic conditions such as psychiatric disorders and pain syndromes. Many antiepileptic drugs (AEDs) have a narrow therapeutic range thus relatively modest alterations of drug concentration in plasma can lead to loss response or toxic effects. Therefore, it is necessary to simultaneously determine AEDs including their active metabolite to improve treatments without undesirable side effects.

Highlights

Simultaneous measurement the concentrations of 21 antiepileptic drugs.

Total run time is 10.5 min.

Just a few pipetting steps for the sample treatment-protein precipitate and shoot approach-no need for derivatization.

Each drug safeguarded by its own internal standard.

Consuming small volume of patient’s sample.

Long life span of HPLC column.

Parameters

Levetiracetam, Lamotrigine, Phenobarbital, Valproic Acid, Oxcarbazepine and its active metabolite named 10-Hydroxycarbamazepine, Carbamazepine Epoxide, N-Desmethylmethsuximide, Topiramate, Carbamazepine, Felbamate, Gabapentine, Lacosamide, Phenytoin, Pregabaline, Rufinamide, Zonisamide, Primidone, Ethosuximide, Tiagabine and Stiripentol

Matrix

Serum/Plasma

Sample Preparation

  • Step 1
    Pipette 50 µL of serum/plasma patient’s sample (calibrator/control) into a glass centrifuge tube.
  • Step 2
    Add 30 µL of internal standard solution and vortex for 5 sec.
  • Step 3
    Add 400 µL of Reagent-1, vortex for 5 sec. then, centrifuge at 4000 rpm for 5 min.
  • Step 4
    Decant the clear supernatant into HPLC vial prior to injection.

Chromatogram

METHOD PERFORMANCE

Analyte LOQ (mg/L)   6460 QQQ LOQ (mg/L)   6470 QQQ LOQ (mg/L)   Ultivo QQQ Accuracy Repeatabilty Linearity
% % CV R2
6470 QQQ 6470 QQQ 6470 QQQ
Carbamazepine 0,87 0,1 0,24 106,83 0,86 0,999
Carbamazepine10,11-epoxide 0,46 0,4 23,11 112,75 0,95 0,999
Ethosuximide – 2,07 1,42 105,06 2,5 0,999
Felbamate 3,88 1,32 1,57 106,86 1,69 0,999
Gabapentin 0,42 0,09 0,29 104,27 0,92 0,999
Lacosamide 0,36 0,13 0,1 106,91 1,08 0,999
Lamotrigine 1,09 0,27 0,24 114,6 1,92 0,998
Levetiracetam 2,92 0,49 1,97 105,47 1,08 0,999
N-Desmethylmethsuximide 3,36 1,29 1,83 106,17 8,55 0,999
Oxcarbazepine 0,23 4,11 0,02 95,49 0,95 0,999
Oxcarbazepine Metabolite 2,38 0,14 1,02 107,81 1,9 0,999
Phenobarbital 3,07 1,95 1,42 97,14 6,52 0,998
Phenytoin 0,73 0,49 87,49 115,33 1,88 0,999
Pregabalin 0,41 0,06 0,13 112,6 1,11 0,999
Primidone 0,95 0,3 0,32 119,83 1,2 0,998
Rufinamide 2,31 0,68 2,15 100,86 1,56 0,999
Stiripentol 0,64 0,12 0,06 99,85 0,88 0,999
Tiagabine 0,02 0,01 0,01 99,81 2,79 0,999
Topiramate 0,39 0,17 0,11 97,62 1,92 0,993
Valproic acid 3,74 1,59 2,87 111,59 8,5 0,998
Zonisamide 1,59 0,47 0,56 100,59 1,24 0,999
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