
Antiepileptic Drugs in Serum/Plasma
Antiepileptic Drugs in Serum/Plasma LC-MS/MS Analysis Kit
Epilepsy is defined as seizure disorder induced by surge of electrical activity in the brain. Anticonvulsant or antiepileptic medications encompass a broad range of drugs that are primarily intended to control ongoing or anticipated future seizures as well as being beneficial in non-epileptic conditions such as psychiatric disorders and pain syndromes. Many antiepileptic drugs (AEDs) have a narrow therapeutic range thus relatively modest alterations of drug concentration in plasma can lead to loss response or toxic effects. Therefore, it is necessary to simultaneously determine AEDs including their active metabolite to improve treatments without undesirable side effects.
Highlights
Simultaneous measurement the concentrations of 21 antiepileptic drugs.
Total run time is 10.5 min.
Just a few pipetting steps for the sample treatment-protein precipitate and shoot approach-no need for derivatization.
Each drug safeguarded by its own internal standard.
Consuming small volume of patient’s sample.
Long life span of HPLC column.
Total run time is 10.5 min.
Just a few pipetting steps for the sample treatment-protein precipitate and shoot approach-no need for derivatization.
Each drug safeguarded by its own internal standard.
Consuming small volume of patient’s sample.
Long life span of HPLC column.
Parameters
Levetiracetam, Lamotrigine, Phenobarbital, Valproic Acid, Oxcarbazepine and its active metabolite named 10-Hydroxycarbamazepine, Carbamazepine Epoxide, N-Desmethylmethsuximide, Topiramate, Carbamazepine, Felbamate, Gabapentine, Lacosamide, Phenytoin, Pregabaline, Rufinamide, Zonisamide, Primidone, Ethosuximide, Tiagabine and Stiripentol
Matrix
Serum/Plasma
Sample Preparation
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Step 1Pipette 50 µL of serum/plasma patient’s sample (calibrator/control) into a glass centrifuge tube.
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Step 2Add 30 µL of internal standard solution and vortex for 5 sec.
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Step 3Add 400 µL of Reagent-1, vortex for 5 sec. then, centrifuge at 4000 rpm for 5 min.
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Step 4Decant the clear supernatant into HPLC vial prior to injection.

Chromatogram
METHOD PERFORMANCE
Analyte | LOQ (mg/L) 6460 QQQ | LOQ (mg/L) 6470 QQQ | LOQ (mg/L) Ultivo QQQ | Accuracy | Repeatabilty | Linearity |
% | % CV | R2 | ||||
6470 QQQ | 6470 QQQ | 6470 QQQ | ||||
Carbamazepine | 0,87 | 0,1 | 0,24 | 106,83 | 0,86 | 0,999 |
Carbamazepine10,11-epoxide | 0,46 | 0,4 | 23,11 | 112,75 | 0,95 | 0,999 |
Ethosuximide | – | 2,07 | 1,42 | 105,06 | 2,5 | 0,999 |
Felbamate | 3,88 | 1,32 | 1,57 | 106,86 | 1,69 | 0,999 |
Gabapentin | 0,42 | 0,09 | 0,29 | 104,27 | 0,92 | 0,999 |
Lacosamide | 0,36 | 0,13 | 0,1 | 106,91 | 1,08 | 0,999 |
Lamotrigine | 1,09 | 0,27 | 0,24 | 114,6 | 1,92 | 0,998 |
Levetiracetam | 2,92 | 0,49 | 1,97 | 105,47 | 1,08 | 0,999 |
N-Desmethylmethsuximide | 3,36 | 1,29 | 1,83 | 106,17 | 8,55 | 0,999 |
Oxcarbazepine | 0,23 | 4,11 | 0,02 | 95,49 | 0,95 | 0,999 |
Oxcarbazepine Metabolite | 2,38 | 0,14 | 1,02 | 107,81 | 1,9 | 0,999 |
Phenobarbital | 3,07 | 1,95 | 1,42 | 97,14 | 6,52 | 0,998 |
Phenytoin | 0,73 | 0,49 | 87,49 | 115,33 | 1,88 | 0,999 |
Pregabalin | 0,41 | 0,06 | 0,13 | 112,6 | 1,11 | 0,999 |
Primidone | 0,95 | 0,3 | 0,32 | 119,83 | 1,2 | 0,998 |
Rufinamide | 2,31 | 0,68 | 2,15 | 100,86 | 1,56 | 0,999 |
Stiripentol | 0,64 | 0,12 | 0,06 | 99,85 | 0,88 | 0,999 |
Tiagabine | 0,02 | 0,01 | 0,01 | 99,81 | 2,79 | 0,999 |
Topiramate | 0,39 | 0,17 | 0,11 | 97,62 | 1,92 | 0,993 |
Valproic acid | 3,74 | 1,59 | 2,87 | 111,59 | 8,5 | 0,998 |
Zonisamide | 1,59 | 0,47 | 0,56 | 100,59 | 1,24 | 0,999 |