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Fatty Acid Profile, Comprehensive in Serum/Plasma GC-MS Analysis Kit

Fatty acid profile is one of the factors affecting health values. Besides the fatty acid composition, the ratios between saturated, monounsaturated, and polyunsaturated fatty acids (SFA, MUFA and PUFA, respectively) and trans fatty acids play an important role. In the examination of the fatty acid profile, the deficiency/high level of fatty acids plays an important role in the diagnosis and treatment of diseases caused by fatty acid oxidation disorders (FAO) and peroxisomal disorders. Fat deficiencies in the body are usually caused by inadequate dietary lipid intake due to unbalanced nutrition, prolonged parenteral nutrition, or intestinal malabsorption. Major clinical manifestations associated with FAO include hypoketotic hypoglycemia, liver disease and failure, skeletal myopathy, dilated/hypertrophic cardiomyopathy, and sudden death. Disease-specific characteristic patterns of metabolites resulting from FAO disorders can be detected in blood, bile, urine, and cultured fibroblasts of many living and dying individuals. Quantitative determination of C8-C18 fatty acids is an important element of the study and differential diagnosis of candidate patients. With the fatty acid profile, patients can detect abnormalities when they are asymptomatic and under diet therapy. In peroxisomal disorders, increased serum levels of very long chain fatty acids (VLCFA, C22:0, C24:0, C26:0), pristanic and phytanic acids indicate Zellweger syndrome.

  • Highlights

    Total run time is 33.4 min.

    The Jasem method accurately analyses esterified fatty acids in the serum/plasma with single sample preparation

    Consuming small volume of patient’s sample

    Long life span of GC column
  • Parameters

    Capric acid, lauric acid, myristoleic acid , myristic acid, palmitoleic acid, palmitic acid,
    gamma-linolenic acid, branched phytanic acid, linoleic acid, alpha-linolenic acid, oleic acid,
    elaidic acid, stearic acid, conjugated linoleic acid, arachidonic acid, eicosapentaenoic acid,
    8,11,14-eicosatrienoic acid, 11,14-eicosadienoic acid, eicosenic acid, 11,14,17-eicosatrienoic
    acid, arachidic acid, docosahexaenoic acid, docosapentaenoic acid, erucic acid, behenic acid,
    nervonic acid, lignoceric acid, cerotic acid.*The number
  • Matrix


Sample Preparation

  • Step 1
    Take 100 μl serum sample into a glass vial
  • Step 2
    Reagent mix prepare into the volumetric flask on ice in fume hood. Reagent 3 is added drop-wise while swirling on R1 and R2, vortex for 10 sec.
  • Step 3
    300 μl of Reagent-mix, vortex for 15 sec.
  • Step 4
    Put the vial into the empty glass tube. İncubate in water bath at 90 min 70°C
  • Step 5
    After cooling to room temperature, the methyl derivatives are transferred in eppendorf, extracted 800 μl Reagent 4 and centrifuge at 3500rpm 2 min.
  • Step 6
    Pipet 600 μl extracted serum samples are dried under stream of nitrogen, dissolved 150 μl Reagent 4, and vortex 10 sec.
  • Step 7
    Transfer the final solution into the insert of HPLC vials
  • Chromatogram

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