Skip to main content
Multi-Mycotoxin In Animal Food

Multi-Mycotoxin In Animal Food LC-MS/MS Analysis Kit

Mycotoxins are secondary metabolites produced by molds, without biochemical significance in fungal growth and development. Although they have existed since ancient times, the term mycotoxins appeared in 1960 when 100 000 turkey poults died due to the ingestion of peanut contaminated with secondary metabolites from Aspergillus flavus.Mycotoxins are compounds with various chemical structures and, therefore, various biological effects in animals and humans such as toxic, carcinogenic, estrogenic, mutagenic, teratogenic, and immunotoxic effects.In Europe, Commission Regulation (EC) No 1881/2006 and its amendments set maximum residue levels (MRLs) for 13 mycotoxins.Jasem multi-mycotoxin method analyzes 10 mycotoxins in one injection and HT-2 and DON are analyzed in second injection with same sample, same column and same instrument parameters except HPLC program.

Highlights

Quick and simple sample preparation steps without immunoaffinity column extraction or evaporation.

Parameters

AF B1, AF B2, AF G1, AF G2, FB1, FB2, OTA, DON, ZON, HT2, T2, STE

Matrix

Animal Food

Sample Preparation


  • Step 1
    Weigh 2 g of animal feed sample into a 50 ml polypropylene falcon.
  • Step 2
    Add 10 ml Reagent 1 onto sample and shake for 15 minutes using multiple-shaker.
  • Step 3
    Centrifuge at 3000 rpm for 5 min.
  • Step 4
    Filter the clear supernatant from 0.45 µm nylon filter into a HPLC vial.

Chromatogram

METHOD PERFORMANCE

Analytes

LOQ (µg/L) 6460 QQQ

Accuracy (%) 6460 QQQ

Repeatability (%RSD) 6460 QQQ

Linearity (R2) 6460 QQQ

AFB1

0.95

93.15

5.06

0.999

AFB2

0.94

91.7

8.58

0.998

AFG1

0.96

79.77

8.53

0.997

AFG2

0.87

86.6

11.98

0.998

DON

378.48

75.55

3.19

0.996

FB1

23.92

116.85

3.06

0.999

FB2

11.47

110.57

7.1

0.995

HT2

120.46

88.99

3.14

0.999

OTA

13.33

101.54

1.62

0.997

STE

1.55

101.45

8.42

0.994

T2

243.18

88.02

1.74

0.999

ZON

23.06

83.22

4.21

0.999

ADAPTING DIAGNOSTICS IN CHROMATOGRAPHY COUPLED MASS SPECTROMETRY..
PRODUCTS
Contact Us

The easiest way is to fill the contact form. Simply mail us to info@jasem.com.tr or give us a call +902165720200